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http://dx.doi.org/10.3839/jabc.2021.011

Protective effects of Carthamus tinctorius L. seed on C6 glial cells treated with ethanol  

Choi, Seung Hak (Department of Physiology, College of Medicine, Inje University)
Park, Chan Hum (Department of Herbal Crop Research, NIHHS, RDA)
Cho, Eun Ju (Department of Food Science and Nutrition, Pusan National University)
Kim, Ji Hyun (Department of Food Science, Gyeongnam National University of Science and Technology)
Seo, Weon Taek (Department of Food Science, Gyeongnam National University of Science and Technology)
Publication Information
Journal of Applied Biological Chemistry / v.64, no.1, 2021 , pp. 69-74 More about this Journal
Abstract
Chronic alcohol is responsible for oxidative stress and neurodegenerative diseases such as dementia. In the present study, we investigated the antioxidant activity and protective effects of seed of Carthamus tinctorius L. on ethanol-induced C6 glial cells. Antioxidant effect of seed of C. tinctorius L. was measured by scavenging activity of 1,1-diphenyl-2-prcrylhydrazy (DPPH), hydroxyl radical (·OH), superoxide radical, and nitric oxide. The seed of C. tinctorius L. extract showed significant radical scavenging activities in a concentration-dependent manner. In particular, it revealed strong DPPH and ·OH scavenging activity, displaying more than 80% at 500 and 100 ㎍/mL, respectively. Treatment of 500 mM ethanol to C6 glial cell led to decline of cell viability and elevation of reactive oxygen species (ROS) generation. However, seed of C. tinctorius L.-treated groups significantly increased cell viability and decreased ROS levels, compared to ethanol-induced control group. These results suggest that seed of C. tinctorius L. would have protective effect against neuronal oxidative stress induced by alcohol.
Keywords
Antioxidant; Carthamus tinctorius L. seed; Ethanol; Glial cells; Reactive oxygen species;
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Times Cited By KSCI : 2  (Citation Analysis)
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