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http://dx.doi.org/10.3839/jabc.2021.007

Construction of novel promoters based on the characteristics of drought stress specific cis-regulatory element  

Kim, Kihwan (Department of Applied Biosciences, Kyungpook National University)
Kim, Byeonggyu (Department of Integrative Biology, Kyungpook National University)
Shin, Juhyung (Department of Integrative Biology, Kyungpook National University)
Kim, Won-Chan (Department of Applied Biosciences, Kyungpook National University)
Publication Information
Journal of Applied Biological Chemistry / v.64, no.1, 2021 , pp. 39-48 More about this Journal
Abstract
Droughts are one of the abiotic stresses that hinders the growth and productivity of crop plants. Coping with abiotic stress is necessary to understand the molecular regulatory networks that makes plants respond to adverse environmental conditions. In our experiment to find a combination that can cope with abiotic stress (respond to drought), we screened 5 stress-inducible promoters that are expressed only under stress conditions. This founded 36 cis-elements in stress-inducible promoters. With the result we designed 2 synthetic promoters (BL1, BL2) for fine-controlled regulation by assembling cis-elements from the native promoters, which are expressed only under stress caused by droughts. Analysis of the transgenic plant (BL1-GUS, BL2-GUS) showed that the synthetic promoters increased the expression of β-glucuronidase (GUS) in transgenic plants under desiccation. Also in the transient activation assay demonstrated that synthetic promoters induced the co-transformation of effector DREB1A and DREB2C. These results expect that the synthetic promoter with a combination of drought-specific elements can be used to respond to various abiotic stress and is resistant to stress without causing growth retardation.
Keywords
Cis-regulatory element; Drought; Fine-controlled regulation; Synthetic promoter;
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