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http://dx.doi.org/10.3839/jabc.2019.055

Isolation and quantitative analysis of metabolites from Scrophularia buergeriana and their hepatoprotective effects against HepG2 Cells  

Na, Hyeon Seon (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Oh, Seon Min (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Shin, Woo Cheol (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Bo, Jeon Hwang (Graduate School of Biotechnology and Department of Oriental Medicine Biotechnology, Kyung Hee University)
Kim, Hyoung-Geun (Graduate School of Biotechnology and Department of Oriental Medicine Biotechnology, Kyung Hee University)
Yoon, Dahye (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Yang, Seung Hwan (Department of Biotechnology, Chonnam National University)
Lee, Young-Seob (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Kim, Geum-Soog (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Baek, Nam-In (Graduate School of Biotechnology and Department of Oriental Medicine Biotechnology, Kyung Hee University)
Lee, Moon-Soon (Department of Industrial Plant Science and Technology, Chungbuk National University)
Lee, Dae Young (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Publication Information
Journal of Applied Biological Chemistry / v.62, no.4, 2019 , pp. 399-406 More about this Journal
Abstract
The roots of Scrophularia buergeriana were extracted with 80% aqueous Methanol and the concentrates were partitioned into EtOAc, n-BuOH, and H2O fractions. The repeated silica gel or octadecyl SiO2column, and medium pressure liquid chromatographies for the n-BuOH fraction led to isolation of phenylethanoid glycosides and iridoid glycosides. The chemical structures of these compounds were determined as harpagoside (1), angoroside C (2), aucubin (3) and acetoside (4) based on spectroscopic analyses including nuclear magnetic resonance and MS. A simple and efficient HPLC with UV detection method for the simultaneous determination of the four compounds (1-4) has been developed and applied to their content determination in the S. buergeriana. The roots were extracted by 80% methanol, and the contents of 1, 2, 3, and 4 were determined to 11.5, 7.6, 41.2, and 4.8 mg/g, respectively. Additionally, angoroside C (2) and acetoside (4) exhibited hepatoprotective effect against ethanol-induced hepatotoxicity in HepG2 cell line.
Keywords
HepG2; Iridoid; Phenylethanoid; Quantitative analysis; Scrophularia buergeriana;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
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