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http://dx.doi.org/10.3839/jabc.2019.050

Optimization of bioactive isorhamnetin 3-O-glucoside production in Escherichia coli  

Kim, Bong-Gyu (Department of Forest Resources, Gyeongnam National University of Science and Technology)
Publication Information
Journal of Applied Biological Chemistry / v.62, no.4, 2019 , pp. 361-366 More about this Journal
Abstract
Isorhamnetin 3-O-glucoside, a member of the flavonol group, has been reported to be effective for inflammatory and ulcer, as well as to alleviate diabetic complications such as neuropathy, nephropathy and retinopathy. Isorhamnetin 3-O-glucoside has been extracted from several plants. Biotransformation is a valuable tool, which is used to produce value-added chemicals with inexpensive compounds. To synthesis isorhamnetin 3-O-glucoside from quercetin, two genes (PGT E82L and ROMT-9) were introduced into Escherichia coli, respectively. In order to synthesis isorhamnetin 3-O-glucoside from quercetin, a co-culture fermentation system was developed by optimizing the medium and temperature for biotransformation, the cell mix ratio, Isopropyl-β-ᴅ-thiogalactoside induction time, and quercetin feed concentration. Finally, isorhamnetin 3-O-glucoside was biosynthesized up to 181.2 mg/L under the optimized biotransformation condition, which was higher 4.7 times than previously reported (39.6 mg/L).
Keywords
Biotransformation; Co-culture; Flavonoids; O-Methyltransferase; UDP-glycosyltransferase;
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