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http://dx.doi.org/10.3839/jabc.2019.001

Biosynthesis of bioactive isokaemferide from naringenin in Escherichia coli  

Kim, Bong-Gyu (Department of Forest Resources, Gyeongnam National University of Science and Technology)
Publication Information
Journal of Applied Biological Chemistry / v.62, no.1, 2019 , pp. 1-6 More about this Journal
Abstract
The flavonoid, isokaempferide, has various biological activities such as hepatoprotective, antimicrobial and antiproliferative effect and is extracted from Amburana cearensis and Cirsium rivulare (Jacq.). Biotransformation is an alternative tool for the synthesis of value-added flavonoids with inexpensive substrates. Here, to synthesize isokaempferide from naringenin, two genes, PFLS and Rice O-mthyltransferae-9 were introduced in Escherichia coli. Although isokaempferide was successfully synthesized, the amount of biosynthesis was no high. In order to increase the yields of isokaempferide, S-adenosylmethionine (SAM) used as a methyl donor was increased by deleting MetJ, which is a transcriptional regulator related to SAM biosynthetic pathway. Next we optimized the cell concentration and substrate feed concentration with the engineered E. coli strain. Through these strategies, the biosynthesis of isokaempferide was increased up to 87 mg/L.
Keywords
Biotransformation; Flavonoids; Flavonol synthase; Metabolic engineering; O-methyltransferase;
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