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http://dx.doi.org/10.3839/jabc.2018.057

Changes in pathogenic characters of Pseudomonas tolaasii 6264 strain by storage period  

Yun, Yeong-Bae (Department of Environmental and Biological Chemistry, Chungbuk National University)
Huh, Jeong-Hun (Department of Environmental and Biological Chemistry, Chungbuk National University)
Kim, Young-Kee (Department of Environmental and Biological Chemistry, Chungbuk National University)
Publication Information
Journal of Applied Biological Chemistry / v.61, no.4, 2018 , pp. 405-410 More about this Journal
Abstract
Pseudomonas tolaasii strain No. 6264 has been isolated from mushroom tissue and identified as one of the major pathogen causing brown blotch disease. It secretes peptide toxins, known as tolaasin and its analogue peptides. P. tolaasii 6264 has been used as a typical pathogenic strain to study the brown blotch disease for last 20 years after confirming its blotch-forming ability, hemolytic activity, and white line formation. In this study, the characteristics of P. tolaasii 6264 strain were analyzed and compared according to storage period. Strains of P. tolaasii 6264 stored annually since 2012 were cultured and their pathogenic characters were analyzed. When the 16S rRNA sequences were compared, all strains were divided into two groups. Pathogenic characters including hemolytic activity, blotch-forming ability, and white line test were also investigated. The strains, P. tolaasii 6264-15-2 and P. tolaasii 6264-17, had all three activities; however, the rest of stored strains showed only blotch-forming ability losing other pathogenic characters. Tolaasin peptides were purified from the bacterial cultures and analyzed by mass spectrometry. The strains, P. tolaasii 6264-15-2 and P. tolaasii 6264-17, secreted Tol I (1987 Da), Tol II (1943 Da), and its analogues (1973 Da, 2005 Da) while some of these peptides were not found in the media cultured other strains. These results indicate that the pathogenicity of P. tolaasii could be varied during the storage period.
Keywords
Brown blotch disease; Mass spectroscopy; Peptide composition; Storage period; Tolaasin;
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1 Lee GS, Byun HS, Yoon KH, Lee JS, Choi KC, Jeung EB (2009) Dietary calcium and vitamin D2 supplementation with enhanced Lentinula edodes improves osteoporosis-like symptoms and induces duodenal and renal active calcium transport gene expression in mice. Eur J Nutr 48: 75-83   DOI
2 Shi YL, James AE, Benzie IFF, Buswell JA (2002) Mushroom-derived preparations in the prevention of H2O2-induced oxidative damage to cellular DNA. Teretog Carcinog Mutagen 22: 103-111   DOI
3 Chen S, Oh SR, Phung S, Hur G, Ye JJ, Kwok SL, Shrode GE, Melury M, Adams LS, Williams D (2006) Anti-aromatase activity of phytochemicals in white button mushrooms (Agaricus bisporus). Cancer Res 66: 12026-12034   DOI
4 Zhang M, Huang J, Xie X, Holman J (2009) Dietary intakes of mushrooms and green tea combine to reduce the risk of breast cancer in Chinese women. Int J Cancer 124: 1404-1408   DOI
5 Annual Report for Production of Mushroom (2016) Agricultural information and statistics. Ministry of Agricultural and Forestry, Sejong
6 Silva T, Claro B, Silva BFB, Vale N, Gomes P, Gomes MS, Funari SS, Teixeira J, Uhrikova D, Bastos M (2018) Unravelling a mechanism of action for a cecropin A-melittin hybrid antimicrobial peptide: the induced formation of multilamellar lipid stacks. Langmuir 34: 2158-2170   DOI
7 Jourdan F, Lazzaroni S, Mendez BL, Lo Cantore P, de Julio M, Amodeo P, Iacobellis NS, Evidente A, Motta A (2003) A left-handed ${\alpha}$-helix containing both L- and D-amino acids: the solution structure of the antimicrobial lipodepsipeptide tolaasin. Proteins 52: 534-543   DOI
8 Hiep HM, Endo T, Saito M, Chikae M, Kim DK, Yamamura S, Takamura Y, Tamiya E (2008) Label-free detection of melittin binding to a membrane using electrochemical-localized surface plasmon resonance. Anal Chem 80: 1859-1864   DOI
9 Bak M, Bywater RP, Hohwy M, Thomsen JK, Adelhorst K, Jakobsen HJ, Sorensen OW, Nielsen NC (2001) Conformation of alamethicin in oriented phospholipid bilayers determined by 15N solid-state nuclear magnetic resonance. Biophys J 81: 1684-1698   DOI
10 Yamaguchi S, Huster D, Waring A, Lehrer RI, Kearney W, Tack BF, Hong M (2001) Orientation and dynamic of an antimicrobial peptide in the lipid bilayer by solid-state NMR spectroscopy. Biophys J 81: 2203-2214   DOI
11 Nutkins JC, Mortishire-Smith RJ, Packman LC, Brodey CL, Rainey PB, Johnstone K, Williams DH (1991) Structure determination of tolaasin, an extracellular lipodepsipeptide produced by the mushroom pathogen Pseudomonas tolaasii paine. J Am Chem Soc 113: 2621-2627   DOI
12 Cho KH, Kim ST, Kim YK (2007) Purification of a pore-forming peptide toxin, tolaasin, produced by Pseudomonas tolaasii 6264. J Biochem Mol Biol 40: 113-118
13 Yun YB, Park SW, Cha JS, Kim YK (2013) Biological characterization of various strains of Pseudomonas tolaasii that causes brown blotch disease. J Korean Soc Appl Biol Chem 56: 41-45   DOI
14 Bassarello C, Lazzaroni S, Bifulco G, Lo Cantore P, Iacobellis NS, Riccio R, Gomez-Paloma L, Evidente A (2004) Tolaasins A-E, five new lipodepsipeptides produced by Pseudomonas tolaasii. J Nat Prod 67: 811-816   DOI
15 Mu LL, Yun YB, Park SJ, Cha JS, Kim YK (2015) Various pathogenic Pseudomonas strains that cause brown blotch disease in cultivated mushrooms. J Appl Biol Chem 58: 349-354   DOI
16 Kim MC, Chun JS (2014) 16S rRNA gene-based identification of bacteria and archaea using the EzTaxon server. In: Goodfellow M (ed) New approaches to prokaryotic systematics, 1st edn. Elsevier, Oxford, pp 61-74
17 Lo Cantore P, Lazzaroni S, Coraiola M, Dalla Serra M, Cafarchia C, Evidente A, Iacobellis NS (2006) Biological characterization of white line-inducing principle (WLIP) produced by Pseudomonas reactans NCPPB1311. MPMI 19: 1113-1120   DOI
18 Burlinson P, Studholme D, Cambray-Young J, Heavens D, Rathjen J, Hodgkin J, Preston GM (2013) Pseudomonas fluorescens NZ17 repels grazing by C. elegans, a natural predator. ISME J 7: 1126-1138   DOI
19 Shirata A, Sugaya K, Takasugi M, Monde K (1995) Isolation and biological activity of toxins produced by a Japanese strain of Pseudomonas tolaasii, the pathogen of bacterial rot of cultivated oyster mushroom. Ann Phytopathol Soc Jpn 61: 492-502
20 Somerville GA, Proctor RA (2009) At the crossroads of bacterial metabolism and virulence factor synthesis in staphylococci. Microbiol Mol Biol Rev 73: 233-248   DOI
21 Rutherford ST, Bassler BL (2012) Bacterial quorum sensing: its role in virulence and possibilities for its control. Cold Spring Harb Perspect Med 2: a012427
22 Wang X, Yue J, Ding C, Wang S, Liu B, Tian M, Yu S (2016) Deletion of AS87_03730 gene changed the bacterial virulence and gene expression of Riemerella anatipestifer. Sci Rep 6: 22438. doi:10.1038/srep22438   DOI
23 Wu YS, Ngai SC, Goh BH, Chan KG, Lee LH, Chuah LH (2017) Anticancer activities of surfactic and potential application of nanotechnology assisted surfactic delivery. Front Pharmocol 8: 761. doi: 10.3389/fphar2017.00761   DOI
24 Kim JK (2009) Disruption of genes related tolaasin biosynthesis by transposon mutagenesis of Pseudomonas tolaasii. Dissertation, Gyeongsang National University
25 Fakruddin M (2012) Biosurfactant: production and application. J Pet Environ Biotechnol 3: 124. doi: 10.4172/2157-7463.1000124