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http://dx.doi.org/10.3839/jabc.2018.001

Screening test for Dendropanax morbifera Leveille extracts: in vitro comparison to ox-LDL-induced lipid accumulation, ethanol-induced fatty liver and HMG-CoA reductase inhibition  

Youn, Ji Sun (Department of Food Science and Technology, Seoul National University of Science and Technology)
Kim, Min Seo (Department of Food Science and Technology, Seoul National University of Science and Technology)
Na, Hye Jin (Korea Food Information Institute)
Jung, Hae Rim (Korea Food Information Institute)
Song, Chang Khil (Agency for Jeju Plant Resources Dendropanax)
Kang, So Young (Agency for Jeju Plant Resources Dendropanax)
Kim, Ji Yeon (Department of Food Science and Technology, Seoul National University of Science and Technology)
Publication Information
Journal of Applied Biological Chemistry / v.61, no.1, 2018 , pp. 1-8 More about this Journal
Abstract
The objective of this study was to compare the antihyperlipidemic effects of different Dendropanax morbifera leaf extracts in vitro. The extracts differed in terms of specimen age, harvesting season, and extraction method. RAW 264.7 cells were pretreated with these extracts and stimulated by oxidized low-density lipoprotein. Ethanol was used to induce toxicity in HepG2 cells. Cellular lipid accumulation was quantified using oil red O staining in both these cells. The extracts were evaluated for their inhibitory effects on 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase. RAW 264.7 cells treated with the 60% ethanol extract of an 8-year-old specimen harvested in November exhibited the lowest lipid accumulation. The 30% ethanol extract of a 5-year-old specimen harvested in May exhibited the greatest protection from ethanol-induced cytotoxicity in HepG2 cells. The hot water extract of an 8-year-old specimen harvested in May showed the greatest inhibition of HMG-CoA reductase. These results showed that D. morbifera extracts prepared from leaves that are harvested in May possess the highest antihyperlipidemic effects.
Keywords
Dendropanax morbifera; 3-hydroxy-3-methylglutaryl coenzyme A reductase; Hyperlipidemia; Lipid accumulation; Oxidized low density lipoprotein;
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