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http://dx.doi.org/10.3839/jabc.2016.061

Analysis of polyethoxylated ascorbic acid using spectrophotometry  

Wu, Zhexue (BK21 Plus KNU Multi-Omics based Creative Drug Research Team, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University)
Liu, Kwang-Hyeon (BK21 Plus KNU Multi-Omics based Creative Drug Research Team, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University)
Publication Information
Journal of Applied Biological Chemistry / v.59, no.4, 2016 , pp. 361-364 More about this Journal
Abstract
We developed a spectrophotometric assay method for polyethoxylated ascorbic acidusing 3-ethyl ascorbic acid as standard material. The analytical method was validated by linearity, accuracy, precision, and stability. The coefficient of variation of the precision of the assay was less than 3.4 %. The linearity of the calibration curves in the desired concentration range is good ($r^2$>0.998). 3-Ethyl ascorbic acid and polyethoxylated ascorbic acid were stable in stock solution at room temperature for up to at least 6 h. The developed assay could be used for the content analysis of polyethoxylated ascorbic acid in samples.
Keywords
Accuracy; Polyethoxylatedascorbic acid; Precision; Spectrophotometry; Validation;
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Times Cited By KSCI : 4  (Citation Analysis)
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1 el Naggar GO, Lovell RT (1991) L-ascorbyl-2-monophosphate has equal antiscorbutic activity as L-ascorbic acid but L-ascorbyl-2-sulfate is inferior to L-ascorbic acid for channel catfish. J Nutr 121: 1622-1626   DOI
2 Eo J, Lee KJ (2008) Effect of dietary ascorbic acid on growth and non-specific immune responses of tiger puffer, Takifugu rubripes. Fish & shellfish immunology 25: 611-616   DOI
3 Kang HK, Kim J-H, Jong H, Kim CH (2015) Effects of Dietary Supplementation of Vitamin C and Sea Buckthorn on the Performance and Meat Quality in Old Laying Hens. Korean J Poult 42: 181-189   DOI
4 Kang HK, Kim SH, Kim JH, Kang GH, Yu DJ, Na JC, Kim DW, Seo OS, Kim GH, Kim GH, Park BS (2007) Effects of Dietary Fish Oil, Vitamin E and C Supplementation on DHA Deposition and Shelf-Life in Broiler Chickens. Korean J Poult 34: 259-269   DOI
5 Liu KH, Byoun JY, Sung HJ, Lee HK, Kim K, Lee HS, Kim JH (2001) Simultaneous determination of furathiocarb and metabolites in biological tissues by high-performance liquid chromatography and post-column derivatization. Chromatographia 53: 687-690   DOI
6 Ministry of Agriculture, Food and Rural Affairs (2016) The notice of Ministry of Agriculture, Food and Rural Affairs 2016-168, Sejong
7 Ministry of Food and Drug Safety (2010) Guideline on Bioanalytical Method Validation, Sejong
8 Morisaki K, Ozaki S (1996) Design of novel hybrid vitamin C derivatives:thermal stability and biological activity. Chem Pharm Bull 44: 1647-1655   DOI
9 Park JG, An YS, Sohn SH, Jang IS, Moon YSM (2013) The Effects of Dietary Supplementation of Vitamin C or E on the Expressions of Endoplasmic Reticulum Stress, Lipid and Glucose Metabolism Associated Genes in Broiler Chickens. Korean J Poult 40: 147-155   DOI
10 Olabisi AO, Mahindaratne MP, Wimalasena K (2005) A convenient entry to C2- and C3-substituted gulono-gamma-lactone derivatives from Lascorbic acid. The J Org Chem 70: 6782-6789   DOI
11 Song J-W, Park S-H, Lee C-R, Lee K-J (2013) Effects of Dietary Supplementation of a Citrus By-product on Growth Performance, Innate Immunity and Tolerance of Low Water Temperature in Red Seabream Pagrus major. Kor J Fish Aquat Sci 46: 399-406
12 Song Y-S, Chung B-Y, Cho W-G, Kang S-H (2000) Development of Polyethoxylated Ascorbic Acid as a Whitening Agent. J Soc Cosmet Sci Korea 35: 199-211
13 Zhou Q, Wang L, Wang H, Xie F, Wang T (2012) Effect of dietary vitamin C on the growth performance and innate immunity of juvenile cobia (Rachycentron canadum). Fish Shellfish Immunol 32: 969-975   DOI
14 Wilson RP (1973) Absence of ascorbic acid synthesis in channel catfish, Ictalurus punctatus and blue catfish, Ictalurus frucatus. Comp Biochem Physiol B 46: 635-638   DOI