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http://dx.doi.org/10.3839/jabc.2016.043

Anti-inflammatory effects of the Rubus occidentalis seed extracts on UV-B induced inflammation in HaCat cells  

Kim, Dong-Hee (Traditional Korean Medicine Technology Division R&D Department Applied Product Development Team, National Development Institute of Korean Medicine)
Park, Tae-Soon (Traditional Korean Medicine Technology Division R&D Department Applied Product Development Team, National Development Institute of Korean Medicine)
Son, Jun-Ho (Traditional Korean Medicine Technology Division R&D Department Applied Product Development Team, National Development Institute of Korean Medicine)
Publication Information
Journal of Applied Biological Chemistry / v.59, no.3, 2016 , pp. 247-253 More about this Journal
Abstract
It confirmed the applicability as an anti-inflammatory material from Rubus occidentalis seed (RSE) extract. In HaCaT cells to evaluate the anti-inflammatory potential as a material RSE extract on the activity of the inflammatory factors caused by UVB and $IFN-{\gamma}/TNF-{\alpha}$. We measured the activity of ROS, interleukin-$1{\beta}$ ($IL-1{\beta}$), interleukin-6 (IL-6) and interleukin-8 (IL-8) by ROS-Glo $H_2O_2$ assay and ELISA kit. Our results showed that the RSE extracts inhibit the UVB and $IFN-{\gamma}/TNF-{\alpha}$-induced ROS activities and expression of $IL-1{\beta}$, IL-6 and IL-8 in a dose-dependent manner. Also it was found that inflammatory mediators of the expression of cyclooxygenase-2 (COX-2) inhibition were also brought, the expression of which is increased $PGE_2$ by COX-2 also inhibited. Finally RSE extracts measure the seed expression of filaggrin in the skin barrier, the main factor of the extract could be confirmed to increase the expression of the filaggrin damaged as a result of this concentration-dependent manner. Through this, it was able to confirm that the efficacy RSE extract to protect the inflammation by restoring the damaged layers of the epidermis. Results from more than RSE extract was able to confirm that the extract that has anti-inflammatory effects by improving the inflammation being produced from UVB.
Keywords
Anti-Inflammatory; HaCaT cell; $PGE_2$; cyclooxygenase-2; Rubus occidentalis Seed;
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