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http://dx.doi.org/10.3839/jabc.2016.025

In vivo and In vitro hair growth promotion effects of extract from Glycine soja Siebold et Zucc  

Yang, Jae Chan (Division of Biomedicinal & Cosmetics, College of Sciences & Technology, Mokwon University)
Kim, Bo Ae (Division of Biomedicinal & Cosmetics, College of Sciences & Technology, Mokwon University)
Publication Information
Journal of Applied Biological Chemistry / v.59, no.2, 2016 , pp. 137-143 More about this Journal
Abstract
Hair is a dermal adjunctive organ that protects the body from external physical and chemical stimuli; hair undergoes anagen, catagen, and telogen phases, with hair-loss occurring during the telogen phase. Alopecia is a condition wherein a person undergoes hair-loss far exceeding the normal amount, owing to diverse external factors. Wild beans are rich in isoflavone and amino acids known to prevent hair-loss; compared to cultivated beans, many wild bean species have higher protein content. This study aimed to develop a hair growth promoting solution, with superior hair growth promoting effects and fewer side effects, using naturally obtained Glycine soja Siebold et Zucc (GSSZ) extracts. Seven-week-old C57BL/6N male mice were classified into different experimental groups. Hair growth was observed in GSSZ-treated mice, and compared against that seen in 3 % minoxidil (MXD, positive control)-treated mice. Visual observations revealed a greater reduction in hair-loss in MXD and GSSZ application groups, compared to that in TXN group (hair loss induction using 1 % testosterone). Evaluation using an image analysis software revealed that compared to the positive control, TXN + GSSZ group showed the highest hair growth. TXN + MXD and control groups exhibited similar follicular cell growth, while the hair growth promotion patterns were similar in the negative control (normal), TXN + GSSZ, and TXN groups, as observed via histological analysis. GSSZ did not induce cytotoxicity (even at 2 mg/mL) in keratinocytes and dermal papilla cells; alternately, dermal papilla cell proliferation was activated in a (GSSZ) concentration-dependent manner. Therefore, the GSSZ extract promoted hair growth and increased hair growth-related cell activity, and could therefore be utilized in alopecia treatment.
Keywords
Alopecia; Dermal papilla cells; Glycine soja Siebold et Zucc; Hair growth; Keratinocyte;
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Times Cited By KSCI : 3  (Citation Analysis)
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