Browse > Article
http://dx.doi.org/10.3839/jabc.2015.055

Various Pathogenic Pseudomonas Strains that Cause Brown Blotch Disease in Cultivated Mushrooms  

Mu, Lin-Lin (Department of Environmental and Biological Chemistry, Chungbuk National University)
Yun, Yeong-Bae (Department of Environmental and Biological Chemistry, Chungbuk National University)
Park, Soo-Jin (Department of Environmental and Biological Chemistry, Chungbuk National University)
Cha, Jae-Soon (Department of Plant Medicine, Chungbuk National University)
Kim, Young-Kee (Department of Environmental and Biological Chemistry, Chungbuk National University)
Publication Information
Journal of Applied Biological Chemistry / v.58, no.4, 2015 , pp. 349-354 More about this Journal
Abstract
Brown blotch disease in cultivated mushrooms is caused by Pseudomonas tolaasii, which secretes a lipodepsipeptide, tolaasin. Tolaasin is a pore-forming toxin in the cell membranes, thus destroying the fruiting body structure of mushroom. In this study, we isolated pathogenic bacteria from mushrooms that had symptoms of brown blotch disease. In order to identify these bacteria, their 16S rRNA genes were sequenced and analyzed. Pathogenic bacteria identified as Pseudomonas species were thirty five and classified into five subgroups: P1 to P5. Each subgroup showed different metabolic profile measured by API 20NE kit. Fifty percent of the bacteria were identified as P. tolaasii (P1 subgroup). All five subgroups caused the formation of brown blotches on mushroom tissues and the optimum temperature was 25oC, indicating that they may be able to secrete causal factors, such as tolaasin and similar peptide toxins. These results show that there are at least five different pathogenic Pseudomonas species as blotch-causing bacteria and, therefore, strains from the P2 to P5 subgroups should be also considered and studied as pathogens in order to improve the quality and yield of mushroom production.
Keywords
brown blotch disease; oyster mushroom; pore-forming toxin; Pseudomonas tolaasii; tolaasin;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Liu F, Ooi VE, and Chang ST (1997) Free radical scavenging activities of mushroom polysaccharide extracts. Life Sci 60, 763-6.   DOI
2 Moquet F, Mamoun M, and Olivier JM (1996) Pseudomonas tolaasii and tolaasin: comparison of symptom induction on a wide range of Agaricus bisporus strains. FEMS Microbiol Lett 142, 99-103.   DOI
3 Munsch P, Alatossava T, Marttinen N, Meyer JM, Christen R, and Gardan L (2002) Pseudomonas costantinii sp. nov., another causal agent of brown blotch disease, isolated from cultivated mushroom sporophores in Finland. Int J Syst Evol Microbiol 52, 1973-83.
4 Munoz R, Guieysse B, and Mattiasson B (2003) Phenanthrene biodegradation by an algal-bacterial consortium in two-phase partitioning bioreactors. Appl Microbiol Biotechnol 61, 261-7.   DOI
5 MushWorld (2004) In Oyster Mushroom Cultivation, Mushroom Grower's Handbook 1, Muchworld, Korea.
6 Nie ZJ, Hang BJ, Cai S, Xie XT, He J, and Li SP (2011) Degradation of cyhalofop-butyl (CyB) by Pseudomonas azotoformans strain QDZ-1 and cloning of a novel gene encoding CyB-hydrolyzing esterase. J Agric Food Chem 59, 6040-6.   DOI
7 Nutkins JC, Mortishire-Smith RJ, Packman LC, Brodey CL, Rainey PB, Johnstone K et al. (1991) Structure determination of tolaasin, an extracellular lipodepsipeptide produced by mushroom pathogen, Pseudomonas tolaasii paine. J Am Chem Soc 113, 2621-7.   DOI
8 Patel S and Goyal A (2012) Recent development in mushrooms as anti-cancer therapeutics: a review. 3 Biotech 2, 1-15.
9 Rainey PB, Brodey CL, and Johnstone K (1991) Biological properties and spectrum of activity of tolaasin, a lipodepsipeptide toxin produced by the mushroom pathogen Pseudomonas tolaasii. Physiol Mol Plant Pathol 39, 57-70.   DOI
10 Annette FM, Zhontang Y, and William WM (2001) Population dynamics and metabolic activity of Pseudomonas abietaniphila BKME-9 within pulp mill wastewater microbial communities assayed by competitive PCR and RT-PCR. FEMS Microbiol Ecol 38, 21-31.   DOI
11 Annual Report for Production of Mushroom (2010) Agricultural information and statistics. Ministry of Agricultural and Forestry, Korea.
12 Tolaas AG (1915) A bacterial disease of cultivated mushrooms. Phytopathology 5, 51-4.
13 Shirata A, Sugaya K, Takasugi M, and Monde K (1995) Isolation and biological activity of toxins produced by a japanese strain of Pseudomonas tolaasii, the pathogen of bacterial rot of cultivated oyster mushroom. Ann Phytopathol Soc Japan 61, 493-502.   DOI
14 Tamura K, Peterson D, Peterson N, Stecher G, Nei M, and Kumar S (2013) MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30, 2725-9.   DOI
15 Tikilili PV and Chirwa EMN (2009) Microbial degradation of recalcitrant organics from radioactive waste using indigenous cultures of naphthalene degrading bacteria. Chem Eng Trans 18, 845-50.
16 Tsuneda A, Suyama K, Murakami S, and Ohira I (1995) Occurrence of Pseudomonas tolaasii on fruiting bodies of Lentinula edodes formed on Quercus logs. Mycoscience 36, 283-8.   DOI
17 Wong WC and Preece TF (1979) Identification of Pseudomonas tolaasii: the white line in agar and mushroom tissue block rapid pitting tests. J Appl Bacteriol 47, 401-7.   DOI
18 Yun YB, Park SW, Cha JS, and Kim YK (2013) Biological characterization of various strains of Pseudomonas tolaasii that causes brown blotch disease. J Korean Soc Appl Biol Chem 56, 415.
19 Bernheimer AW and Rudy B (1986) Interactions between membranes and cytolytic peptides. Biochem Biophys Acta 864, 123-41.
20 Bassarello C, Lazzaroni S, Bifulco G, Cantore PL, Iacobellis NS, Riccio R et al. (2004) Tolaasins A-E, five new lipodepsipeptides produced by Pseudomonas tolaasii. J Nat Prod 67, 811-6.   DOI
21 Brodey CL, Rainey PB, Tester M, and Johnstone K (1991) Bacterial blotch disease of the cultivated mushroom is caused by an ion channel forming lipodepsipeptide toxin. Mol Plant-Microbe Interact 4, 407-11.   DOI
22 Cho KH and Kim YK (2003) Two types of ion channel formation of tolaasin, a Pseudomonas peptide toxin. FEMS Microbiol Lett 221, 221-6.   DOI
23 Cho KH, Kim ST, and Kim YK (2007) Purification of a pore-forming peptide toxin, tolaasin, produced by Pseudomonas tolaasii 6264. J Biochem Mol Biol 40, 113-8.   DOI
24 Cho KH, Wang HS, and Kim YK (2010) Temperature-dependent hemolytic activity of membrane pore-forming peptide toxin, tolaasin. J Pept Sci 16, 85-90.   DOI
25 Coraiola M, Lo Cantore P, Lazzaroni S, Evidente A, Iacobellis NS, and Dalla Serra M (2006) WLIP and tolaasin I, lipodepsipeptides from Pseudomonas reactans and Pseudomonas tolaasii, permeabilise model membranes. Biochem Biophys Acta 1758, 1713-22.   DOI
26 Fletcher JT (1979) Bacteria and mushrooms. Mushroom J 82, 451-7.
27 Gandy DG (1968) A technique for screening bacteria causing brown blotch of cultivated mushrooms. Annual Report of the Glasshouse Crops Research Institute for 1967, 150-4.
28 Kubo K and Nanba H (1996) The effect of mushrooms on liver and serum lipids. Altern Ther Health Med 2, 62-9.