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http://dx.doi.org/10.3839/jabc.2015.003

Detection of Early Intermediates of the Glycosylphosphatidylinositol anchor in Liquid-cultured Arabidopsis  

Cheong, Jong-Joo (Center for Food and Bioconvergence, Seoul National University)
Kwon, Hawk-Bin (Division of Biomedical Sciences, Sunmoon University)
Publication Information
Journal of Applied Biological Chemistry / v.58, no.1, 2015 , pp. 9-11 More about this Journal
Abstract
Tissue extracts were prepared from liquid-cultured Arabidopsis and reacted with UDP-[$^3H$]-GlcNAc. Phospholipid fractions were then extracted by butanol partitioning. Consecutive thin-layer chromatography identified two glycolipids sensitive to PI-specific phospholipase C, known as early intermediates in glycosylphosphatidylinositol anchor biosynthesis; phosphatidylinositol N-acetylglucosamine and phosphatidylinositol glucosamine.
Keywords
Arabidopsis; glycosylphosphatidylinositol anchor; liquid culture; thin-layer chromatography;
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