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http://dx.doi.org/10.3839/jabc.2014.055

Norsesquiterpenes from the Roots of White Kwao Krua (Pueraria mirifica)  

Kwon, Jung-Hwa (Graduate School of Biotechnology & Department of Oriental Medicine Biotechnology, Kyung Hee University)
Cho, Jin-Gyeong (Graduate School of Biotechnology & Department of Oriental Medicine Biotechnology, Kyung Hee University)
Park, Hee-Jung (Graduate School of Biotechnology & Department of Oriental Medicine Biotechnology, Kyung Hee University)
Huh, Gyu-Won (Graduate School of Biotechnology & Department of Oriental Medicine Biotechnology, Kyung Hee University)
Bang, Myun-Ho (Graduate School of Biotechnology & Department of Oriental Medicine Biotechnology, Kyung Hee University)
Han, Min-Woo (Graduate School of Biotechnology & Department of Oriental Medicine Biotechnology, Kyung Hee University)
Oh, Chang-Hwan (Oriental Medical Food &Nutrition, Semyung University)
Ko, Sung-Kwon (Oriental Medical Food &Nutrition, Semyung University)
Cho, Soo-Yeul (Advanced Analysis Team, National Institute of Food and Drug Safety Evaluation, Korea Food & Drug Administration)
Chai, Kap-Yong (Advanced Analysis Team, National Institute of Food and Drug Safety Evaluation, Korea Food & Drug Administration)
Kim, Jin-Ho (Advanced Analysis Team, National Institute of Food and Drug Safety Evaluation, Korea Food & Drug Administration)
Baek, Nam-In (Graduate School of Biotechnology & Department of Oriental Medicine Biotechnology, Kyung Hee University)
Publication Information
Journal of Applied Biological Chemistry / v.57, no.4, 2014 , pp. 347-352 More about this Journal
Abstract
The roots of Pueraria mirifica were extracted with 70% aqueous ethyl alcohol and partitioned into ethyl acetate (EtOAc), n-butyl alcohol (BuOH), and $H_2O$ fractions, successively. From the EtOAc fraction, four norsesquiterpenes were isolated through the repeated silica gel, octadecyl silica gel and Sephadex LH-20 column chromatographies. On the basis of physicochemical and spectroscopic data including nuclear magnetic resonance (NMR), mass spectrometry, and infrared spectroscopy, the chemical structures were identified as megastigm-5-en-3,9-diol (1), linarionoside B (2), 3,5,6,9-tetrahydroxymegastigm-7-ene (3) and 3,4,9-trihydroxymegastigma-5,7-diene (4). Especially, the configuration of the anomer hydroxyl group was determined as a from the coupling constants of the anomer proton (J =8.0 Hz) in the $^1H$-NMR spectrum. These compounds were isolated for the first time from the roots of P. mirifica in this study.
Keywords
3,4,9-trihydroxy-megastigma-5,7-diene; 3,5,6,9-tetrahydroxy megastigm-7-ene; linarionoside B; megastigm-5-en-3,9-diol; norsesquiterpene; Pueraria mirifica; White Kwao Krua;
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Times Cited By KSCI : 3  (Citation Analysis)
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