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http://dx.doi.org/10.3839/jabc.2014.036

Anti-inflammatory Activity of Sargassum micracanthum Water Extract  

Jeong, Da Hyun (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Kang, Bo Kyeong (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Kim, Koth Bong Woo Ri (Institute of Fisheries Sciences, Pukyong National University)
Kim, Min Ji (Institute of Fisheries Sciences, Pukyong National University)
Ahn, Dong Hyun (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Publication Information
Journal of Applied Biological Chemistry / v.57, no.3, 2014 , pp. 227-234 More about this Journal
Abstract
The anti-inflammatory effect of Sargassum micracanthum water extract (SMWE) was investigated using lipopolysaccharide (LPS)-induced inflammatory response in this study. The murine macrophage cell line RAW 264.7 cells were used and MTT assay was performed to measure the cell proliferation ability. The secretion of nitric oxide (NO), tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), interleukin-6 (IL-6), and IL-$1{\beta}$ was measured in LPS-induced RAW 264.7 cells by ELISA. The expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and nuclear transcription factor-kappa B p65 protein was studied by immunoblotting. The Balb/c mice were used for an acute toxicity test, and imprinting control region mice were purchased to evaluate a croton oil-induced ear edema. As a result, there was no cytotoxicity in the macrophage proliferation treated with SMWE compared to the control. NO levels decreased with increasing concentration of SMWE and were inhibited over 50%. Moreover, the secretion of IL-6, TNF-${\alpha}$, and IL-$1{\beta}$ was suppressed in a dose-dependent manner, especially, IL-$1{\beta}$ inhibition activity was over 50% at 50 ${mu}g$/mL. The formation of ear edema of mice was reduced at the highest dose tested compared to that in the control. Moreover, in acute toxicity test, no moralities occurred in mice administered 5,000 mg/kg body weight of SMWE over 2 weeks observation period. These results suggested that SMWE may have significant effects on inflammatory factors and be potential anti-inflammatory therapeutic materials.
Keywords
anti-inflammation; ear edema; nuclear transcription factor-kappa B(NF-${\kappa}B$); Sargassum micracanthum;
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