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http://dx.doi.org/10.3839/jabc.2013.040

Inhibitory Effect of Sargauum fulvellum Ethanolic Extract on LPS-Induced Inflammatory Reaction in RAW 264.7 Mouse Macrophages  

Kim, Min-Ji (Institute of Fisheries Sciences/Pukyong National University)
Kim, Koth-Bong-Woo-Ri (Institute of Fisheries Sciences/Pukyong National University)
Jeong, Da-Hyun (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Ahn, Dong-Hyun (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Publication Information
Journal of Applied Biological Chemistry / v.56, no.4, 2013 , pp. 249-255 More about this Journal
Abstract
Recently, algae has been considered as a potential anti-inflammatory source due to its distinctive habitat environment exposing to light and high oxygen concentration. In present study, anti-inflammatory effect of brown alga, Sargassum fullvellum ethanol extract (SFEE), was examined. SFEE inhibited not only the production of nitric oxide and pro-inflammatory cytokines (IL-6, IL-$1{\beta}$, TNF-${\alpha}$) but also the expression of inducible nitric oxide synthase and cyclooxygenase 2 in LPS-induced RAW 264.7 cells without affecting cell viability. SFEE also suppressed the expression of nuclear factor kappa B (NF-${\kappa}B$), suggesting that SFEE could affect the expression of inflammation related cytokines and proteins through the regulation of NF-${\kappa}B$. Furthermore, formation of edema of the ear was 40% lower in mice treated with the highest dose (250 mg/kg) of SFEE than in the control mice. Thus, our study showed that SFEE may be a potential therapeutic anti-inflammatory drug.
Keywords
cyclooxygenase 2; ear edema; inducible nitric oxide synthase; nuclear factor kappa B; pro-inflammatory cytokines;
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1 Ahn YW, Lee SH, Choi YH, Kim SK, Yea SS, Choi I et al. (2009) Ecklonia cava ethanolic extracts inhibit lipopolysaccharide-induced cyclooxygenase-2 and inducible nitric oxide synthase expression in BV2 microglia via the MAP kinase and NF-$\kappa{B}$ pathways. Food Chem Toxicol 47, 410-7.   DOI   ScienceOn
2 Amira M, Gamal-Eldeen, Abo-Zeid Mona AM, and Eman F Ahmed (2013) Anti-genotoxic effect of the Sargassum dentifolium extracts: Prevention of chromosomal aberrations, micronuclei, and DNA fragmentation. Exp Toxicol Pathol 65, 27-34.   DOI   ScienceOn
3 Bae SJ (2004) Anticarcinogenic effects of Sargassum fulvellum fractions on several human cancer cell lines in vitro. J Korean Soc Food Sci Nutr 33, 480--6.   과학기술학회마을   DOI   ScienceOn
4 Baldwin Jr AS (1996) NF-kappa B and I kappa B proteins: new discoveries and insights. Annu Rev Immuno 14, 649-83.   DOI   ScienceOn
5 Berenbaum F (2000) Proinflammatory cytokines, prostaglandins, and the chondrocyte: mechanism of intracellular activation. Joint Bone Spine 67, 561-4.   DOI   ScienceOn
6 Bogdan C (2001) Nitiric oxide and the immune response. Nat Immunol 2, 907-16.   DOI   ScienceOn
7 Castrillo A, Diaz-Guerra MJM, Hortelano S, Martin-Sanz P, and Bosca L (2000) Inhibition of I$\kappa{B}$ kinase and I$\kappa{B}$ phosphorylation by 15-deoxy-D12, 14-prostaglandin J2 in activated murine macrophages. Mol Cell Biol 20, 1692-8.   DOI   ScienceOn
8 Chen F, Sun SC, Kuh DC, Gaydos LJ, and Demers LM (1995) Essential role of NF-kappa B activation in silica-induced inflammatory mediator production in macrophages. Biochem Bioph Res Co 214, 985-92.   DOI   ScienceOn
9 Chen HH, Lin HT, Foung YF, and Lin JHY (2012) The bioactivity of teleost IL-6: IL-6 protein in orange-spotted grouper (Epinephelus coioides) induces Th2 cell differentiation pathway and antibody production. Dev Comp Immunol 38, 285-94.   DOI   ScienceOn
10 Chiu CT, Chuang CY, Li JR., Huang HY, Chang SW, and Hung YC (2010) Greater therapeutic efficacy of prednisolone plus medicinal herbs than prednisolone or medicinal herbs alone in patients with oral lichen planus. J Dental Sci 5, 209-15.   DOI   ScienceOn
11 Corker R and Laurent GJ (1998) Pulmonary fibrosis. Cytokines in the balance. Eur Respir J 11: 1218-21.   DOI   ScienceOn
12 Corriveau CC and Danner RL (1993) Endotoxin as a therapeutic target in septic shock. Infect Agent Dis 2, 35-43.
13 Dar A, Baig HS, Saifuallah SM, Ahmad VU, Yasmeen N, and Nizamuddin M (2007) Effect of seaxonal variation on the anti-inflammatory activity of Sargassum sightii growing on the N. Arabian Sea coast of Pakistan. J Exp Mar Boil Ecol 351, 1-9.   DOI   ScienceOn
14 Josephine A, Nithya K, Amudha G, Beena CK, Preetha SP, and Varalakshmi P (2008) Role of sulphated polysaccharides from Sargassum wightii in cyclosporine A-induced oxidative liver injury in rats. BMC Pharmacol 8, 4-13.
15 Esposito E and Cuzzocrea S (2007) The role of nitiric oxide synthases in lung inflammation. Curr Opin Invest Drugs 8, 899-909.
16 Hoe SJ, Yoon WJ, Kim KN, Ahn GN, Kang SM, Kang DH et al. (2010) Evaluation of anti-inflammatory effect of fucoxanthin isolated brown algae in lipopolysaccharide-stimulated RAW 264.7 macrophages. Food Chem Toxicol 48, 2045-51.   DOI   ScienceOn
17 Jin DQ, Lim CS, Sung JY, Choi HG, Ha IH, and Han JS (2006) Ulva conglabata, a marine alga, has neuroprotective and anti-inflammatory effects in murine hippocampal and microglial cells. Neurosci Lett 402, 154-8.   DOI   ScienceOn
18 Kang JY, Khan MN, Park NH, Cho JY, Lee MC, and Fujii H (2008) Antipyretic analgesic, and anti-inflammatory activities of the seaweed Sargassum fulvellum and Sargassum thunbergii in mice. J Ethnopharmacol 116, 187-90.   DOI   ScienceOn
19 Kaplanski G, Marin V, Montero-Julian F, Mantovani A, and Farnarier C (2003) IL-6: A regulator of the transition from neutrophil to monocyte recruitment during inflammation. Trends Immunol 24, 25-9.   DOI   ScienceOn
20 Kim YW, Zhao RJ, Park SJ, Lee JR, Cho IJ, Yang CH et al. (2008) Antiinflammatory effects of liquiritigenin as a consequence of the inhibition of NF-$\kappa{B}$-dependent iNOS and proinflammatory cytokines production. Br J Pharmacol 154, 165-73.   DOI   ScienceOn
21 Kubes P and McCafferty DM (2000) Nitric oxide and intestinal inflammation. Am J Med 109, 150-8.   DOI   ScienceOn
22 Prussin C and Metcalfe DD (2003) IgE, mast cells, basophils, and eosinophils. J allergy Clin Immunol 111, S486-94.   DOI   ScienceOn
23 Li YX, Wijesekara I, Li Y, and Kim SK (2011) Phlorotannins as bioactive agents from brown algae. Process Biochem 46, 2219-24.   DOI   ScienceOn
24 Lowenstein CJ, Alley EW, Rava Pl, Snowman AM, Snyder SH, Russell SWet al. (1993) Macrophage nitiric oxide synthase gene: Two upstream regions mediate induction by interferon gamma and lipopolysaccharide. Proc Natl Acad Sci USA 90, 9730-4.   DOI   ScienceOn
25 Murakami A and Ohigashi H (2007) Targeting NO, iNOS and COX-2 in inflammatory cells: Chemoprevention using food phytochemicals. Int J Cancer 121, 2357-63.   DOI   ScienceOn
26 Renard P and Raes M (1999) The proinflammatory transcription factor NF-$\kappa{B}$: A potential target for novel therapeutical strategies. Cell Biol Toxicol 115, 341-4.
27 Rothwarf DM, Zandi E, Natoli G, and Karin M (1998) IKK-gamma is an essential regulatory subunit of the IkappaB kinase complex. Nature 395, 297-300.   DOI   ScienceOn
28 Shapira L, Soskolne WA, Houri Y, Barak V, Halabi A, and Stabholz A (1996) Protection against endotoxic shock and lipopolysaccharide-induced local inflammation by tetracycline: correlation with inhibition of cytokine secretion. Infect Immun 64, 825-8.
29 Van JR, Mitchell JA, Appleton I, Tomlinson A, Bishop-Bailey D, Croxtall J et al. (1994) Inducible isoforms of cyclooxygenase and nitiric-oxide synthase in inflammation. Proc Natl Acad Sci USA 91, 2046-50.   DOI   ScienceOn
30 Wallace JL (2005) Nitric oxide as a regulator of inflammatory processes. Mem I Oswaldo Cruz 100: 5-9.   DOI
31 Wijesekara I, Yoon NY, and Kim SK (2010) Phlorotannins from Ecklonia cava (Phaeophyceae): biological activities and potential health benefits. Biofactors 36, 408-14.   DOI   ScienceOn
32 Watson WH, Zhao Y, and Chawla RK (1999) S-adenosylmethionine attenuates the lipopolysaccharide-induced expression of the gene for the tumour necrosis factor-a. Biochem J 342(Pt 1), 21-5.   DOI   ScienceOn
33 WHO (1992) Research guidelines for evaluating the safety and efficacy of herbal medicine. Regional office for western pacific, Philiphines.
34 Wijesekara I, Pangestuti R, and Kim SK (2011) Biological activities and potential health benefits of sulfated polysaccharides derived from marine algae. Carbohydr Polym 84, 14-21.   DOI   ScienceOn