Browse > Article
http://dx.doi.org/10.3839/jabc.2012.024

Antibacterial Activity and Protective Role against Gastric Cancer by Sedum sarmentosum  

Choi, Ji Yeon (Department of Food Science and Nutrition, Pusan National University)
Kim, Hye Min (Department of Integrative Plant Science, Chung-Ang University)
Mok, So-Youn (Department of Integrative Plant Science, Chung-Ang University)
Choi, Kyung (Department of Forest Resource Conservation, Korea National Arboretum)
Ku, Jajung (Department of Forest Resource Conservation, Korea National Arboretum)
Park, Kwang-Woo (Department of Forest Resource Conservation, Korea National Arboretum)
Cho, Eun Ju (Department of Food Science and Nutrition, Pusan National University)
Lee, Sanghyun (Department of Integrative Plant Science, Chung-Ang University)
Publication Information
Journal of Applied Biological Chemistry / v.55, no.3, 2012 , pp. 157-161 More about this Journal
Abstract
The aim of this research was to investigate the industrial application of Sedum sarmentosum. Antibacterial activities of the n-hexane, methylene chloride (MC), ethyl acetate, and n-butanol fractions of S. sarmentosum were tested against Escherichia coli, Staphylococcus aureus, and Helicobacter pylori. The MC fraction showed the strongest antibacterial activity against E. coli, with an inhibition zone greater than 13 mm in disc assays. At $100{\mu}g/mL$, all fractions scavenged more than 50% of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radicals (${\cdot}OH$). In particular, the MC fraction showed the strongest scavenging activity against DPPH and ${\cdot}OH$. In addition, we found that treatment with the MC fraction inhibited the growth of H. pylori and gastric adenocarcinoma cells. The present results suggest that the MC fraction of S. sarmentosum would play the promising protective role against pathogenic bacteria and free radicals.
Keywords
antibacterial; anticancer; antioxidant; free radical; Sedum sarmentosum;
Citations & Related Records
Times Cited By KSCI : 7  (Citation Analysis)
연도 인용수 순위
1 Bae JH (2005) Antimicrobial effect of Hedyotis diffusa extracts on food-borne pathogens. J Korean Soc Food Sci Nutr 34, 107-12.   DOI   ScienceOn
2 Carmichael J, DeGraff WG, Gazdar AF, Minna JD, and Mitchell JB (1987) Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res 47, 936-42.
3 Cho YS, Lee J, Lee MK, Shin DB, Kim DH, and Park KM (2011) Prevalence and characterization of Staphylococcus aureus pathogenic factors isolated from various foods in Korea. Korean J Food Sci Technol 43, 648-54.   DOI   ScienceOn
4 Chung SK, Osawa T, and Kawakishi S (1997) Hydroxyl radical-scavenging effects of spices and scavengers from brown mustard (Brassica nigra). Biosci Biotechnol Biochem 61, 118-23.   DOI   ScienceOn
5 Davidson PH and Parish ME (1989) Methods for testing the efficacy of food antimicrobials. Food Technol 43, 148-52.
6 Doyle MP (1991) Escherichia coli O157: H7 and its significance in foods. Int J Food Microb 12, 289-301.   DOI   ScienceOn
7 Fridorich I (1978) The biology of oxygen radicals. Science 201, 875-80.   DOI
8 Gardner PR and Fridovich I (1991) Superoxide sensitivity of the Escherichia coli 6-phosphogluconate dehydratase. J Biol Chem 266, 1478-83.
9 Goldberg I (1994) In Functional Foods: Designer Foods, Pharmafoods, Nutraceuticals. Chapman & Hall Press, USA.
10 Graham DY (1989) Campylobacter pylori and peptic ulcer disease. Gastroenterol 96, 615- 25.   DOI
11 Ham SH, Lee SY, Choi M, and Hwang Bo HJ (1998) Antimutagenicity and cytotoxicity effects of Woorimil wheat flour extracts added with wild herb and seaweed power. J Korean Soc Food Sci Nutr 27, 1177- 82.
12 Hatano T, Edamatsu R, Hiramatsu M, Mori A, Fujita Y, Yasuhara T et al. (1989) Effects of the interaction of tannins with co-existing substances, VI: Effects of tannins and related polyphenols on superoxide anion radical, and on 1,1-diphenyl-2-picrylhydrazyl radical. Chem Pharm Bull 37, 2016- 21.   DOI
13 Isomoto H, Inoue K, Furusu H, Nishiyama H, Shikuwa S, Omagari K et al. (2003) Lafutidine, a novel histamine H2-receptor antagonist, vs lansoprazole in combination with amoxicillin and clarithromycin for eradication of Helicobacter pylori. Helicobacter 8, 111-9.   DOI   ScienceOn
14 He A, Wang M, Hao H, Zhang D, and Lee KH (1998) Hepatoprotective triterpenes from Sedum sarmentosum. Phytochemistry 49, 2607-10.   DOI   ScienceOn
15 Hong J, Wie M, Leem D, Park KS, Yoon T, No KM et al. (2010) Evaluation of antioxidants activity through the chemical assay. J Biomed Res 11, 1- 8.
16 Imlay JA and Linn S (1988) DNA damage and oxygen radical toxicity. Science 240, 1302-9.   DOI
17 Jung HJ, Choi YJ, Won CW, and Seo YR (2006) The chemopreventive effects of antioxidant enzyme. Environ Mutagen Carcinogen 6, 35-64.
18 Kang TH, Pae HO, Yoo JC, Kim NY, Kim YC, Ko GI et al. (2000) Antiproliferative effects of alkaloids from Sedum sarmentosum on murine and human hepatoma cell lines. J Ethnopharmacol 70, 177-82.   DOI   ScienceOn
19 Kim HJ and Lee SY (2007) Genetic relationships based on morphological characteristics in Korean native Sedum sarmentosum. Korean J Hort Sci Technol 25, 103-9.
20 Kim JH, Sung NY, Kwon SK, Jung PM, Choi JI, Yoon YH et al. (2010) Antioxidant activity of Stevia leaf extracts prepared by various extraction methods. J Korean Soc Food Sci Nutr 39, 313-8.   DOI   ScienceOn
21 Kim WH, Bae SJ, and Kim M (2002) The effects of Sedum sarmentosum Bunge on serum lipid concentration in ovariectomized rats. J Korean Soc Food Sci Nutr 31, 290-4.   DOI   ScienceOn
22 Lim JS, Yoon JH, Min BK, and Hong WK (2008) Detection and identification of shiga-like toxin producing E. coli O157: H7 by multiplex PCR. Food Eng Prog 12, 8-14.
23 Kim YD, Mahinda S, Koh KS, Jeon YJ, and Kim SH (2009) Reactive oxygen species scavenging activity of Jeju native citrus peel during maturation. J Korean Soc Food Sci Nutr 38, 462-9.   DOI   ScienceOn
24 Kwack BH (1976) On the ecology of Sedum sarmentosum Bunge in Korea. J Korean Soc Hort Sci 17, 69-77.
25 Lee HJ, Lim GN, Park MA, and Park SN (2011) Antibacterial and antioxidative activity of Lespedeza cuneata G. Don extracts. Korean J Microbiol Biotechnol 39, 63-9.
26 Morrison DM, Tyrrell DL, and Jewell LD (1986) Colonic biopsy in verotoxin-induced hemorrhagic colitis and thrombotic thrombocyropenic purpura (TTP). Am J Clin Pathol 86, 108-12.   DOI
27 Nam SM, Ham SS, Oh DH, Kang IJ, Lee SY, and Chung CK (1998) Effects of Artemisia iwayomogi Kitamura ethanol extract on lowering serum and liver lipids in rats. J Korean Soc Food Sci Nutr 27, 338-43.
28 Novello A (1999) Outbreak of Escherichia coli O157: H7 and Campylobacter among attendees of the Washington County Fair-New York, 1999. MMWR Morb Mortal Wkly Rep 48, 803-5.
29 NSOK (2011) Annual report on the cause of death statistics. National Statistical Office of Korea, Korea.
30 Oh H, Kang DG, Kwon JW, Kwon TO, Lee SY, Lee DB et al. (2004) Isolation of angiotensin converting enzyme (ACE) inhibitory flavonoids from Sedum sarmentosum. Biol Pharm Bull 27, 2035-7.   DOI   ScienceOn
31 Ohta T, Shibata H, Kawamori T, Iimuro M, Sugimura T, and Wakabayashi K (2001) Marked reduction of Helicobacter pylori-induced gastritis by urease inhibitors, acetohydroxamic acid and flurofamide, in Mongolian gerbils. Biochem Biophys Res Commun 285, 728-33.   DOI   ScienceOn
32 Sim GS, Kim JH, Lee BC, Lee DH, Lee GS, and Pyo HB (2008) Inhibitory Effects on Melanin Production in B16 Melanoma Cells of Sedum sarmentosum. Arch Pharm Res 52, 165-71.
33 Parente F, Sainaghi M, Sangaletti O, Imbesi V, Maconi G, Anderloni A et al. (2002) Different effects of short-term omeprazole, lansoprazole or pantoprazole on the accuracy of the $^{13}C$-urea breath test. Aliment Pharmacol Ther 16, 553-7.   DOI   ScienceOn
34 Park S, Kim H, Paek SH, Hong JW, and Kim YK (2008) Enzyme-linked immune-strip biosensor to detect Escherichia coli O157:H7. Ultramicrorscopy 108, 1349-51.
35 Sadaki O (1996) The development of functional foods and materials. Bioindustry 13, 44-50.
36 Sim GS, Kim JH, Lee DH, Na Y, Lee GS, and Pyo HB (2007) Sedum sarmentosum enhances hyaluronan synthesis in transformed human keratinocytes and increases water content in human skin. J Soc Cosmet Scientists Korea 33, 17-22.
37 Waren JR and Marshall B (1983) Unidentified curved bacilli on gastric epithelium in active chronic gastritis. Lancet 321, 1273-5.   DOI   ScienceOn
38 Yoon SJ, Kim JS, Jo BS, Kim JH, Lee SH, Ahn BJ et al. (2011) Isolation and identification of antimicrobial compounds against Helicobacter pylori from Rosemary (Rosmarinus officinalis L.) extracts. J Appl Biol Chem 54, 159-65.   DOI   ScienceOn