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http://dx.doi.org/10.3839/jabc.2011.041

Development of Media for the Cultivation of Enterobacter amnigenus GG0461 and its Nitrate Uptake  

Park, Seong-Wan (Department of Environmental and Biological Chemistry, Chungbuk National University)
Yoon, Young-Bae (Department of Environmental and Biological Chemistry, Chungbuk National University)
Wang, Hee-Sung (Department of Environmental and Biological Chemistry, Chungbuk National University)
Kim, Young-Kee (Department of Environmental and Biological Chemistry, Chungbuk National University)
Publication Information
Journal of Applied Biological Chemistry / v.54, no.4, 2011 , pp. 252-257 More about this Journal
Abstract
To remove excess nitrate from the agricultural environments, Enterobacter amnigenus GG0461 has been isolated as a bacterial strain having high capability of nitrate uptake activity. This strain was able to remove nitrate more than 3,000 ppm (50 mM) in the Pseudomonas agar F (PAF) medium. Therefore, it could be a candidate strain for a nitrate scavenger in the various contaminated environments, such as agricultural soils, livestock sewage, and industrial wastewater. In order to develop medium for the large-scale production of the strain GG0461, each component of PAF medium was replaced with the corresponding commercial product and the optimal conditions for bacterial growth and nitrate uptake activity were measured. Glycerol was replaced with the commercially available product and the nitrogen source was substituted with commercial tryptone, yeast extract, soybean meal, and fermented fish extract. Bacterial growth and nitrate uptake activity were maximal in the media containing 2% tryptone, followed by yeast extract, soybean meal, and fermented fish extract. The pH of the growth medium containing 2% tryptone was decreased by the bacterial nitrate uptake, suggesting that the nitrate uptake is mediated by a nitrate/proton antiporter. This result shows that the medium containing commercial tryptone was good enough for the physiological activity of the strain GG0461. Each component of PAF medium was successfully replaced with the corresponding commercial product except peptone. In conclusion, the composition of medium for the cultivation of the strain GG0461 was determined as 2% tryptone, 1% glycerol, plus required salts according to the composition of PAF medium.
Keywords
commercial media; microbial remediation; nitrogen source; nitrate uptake;
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1 Archibald RD, Harper RJ, Fox JED, and Silberstein RP (2006) Tree performance and root-zone salt accumulation in three dryland Australian plantations. Agroforest Syst 66, 191-204.   DOI   ScienceOn
2 Cha WS, Choi HI, Lee DB, and Cha JM (2003) Isolation and characterization of denitrification bacteria. Korean J Biotechnol Bioeng 18, 461-465.
3 Choi TK, Kim ST, Han MW, and Kim YK (2008) Enhanced nitrate uptake by Enterobacter amnigenus GG0461 at alkaline pH. J Korean Soc Appl Biol Chem 51, 1-5.
4 Crawford NM (1995) Nitrate: Nutrient and signal for plant growth. Plant Cell 7, 859-868.   DOI   ScienceOn
5 de Pascale S, Barbieri G, and Ruggiero C (1997) Effects of water salinity on plant growth and water relations in snap bean(Phaseolus vulgaris L.). Acta Hort 449, 649-655.
6 Drysdale GD, Kasan HC, and Bux F (1999) Denitrification by heterotrophic bacteria during activated sludge treatment. Water SA 25, 357-362.
7 Eker S, Comertpay G, Konuskan O, Ulger AC, Ozturk L, and Cakmak I (2006) Effect of salinity stress on dry matter production and ion accumulation in hybrid maize varieties. Turk J Agric For 30, 365-373.
8 Hasegawa PM, Bressan RA, Zhu JK, and Bohnert HJ (2000) Plant cellular and molecular responses to high salinity. Annu Rev Plant Physiol Plant Mol Biol 52, 463-499.
9 Incharoensakdi A and Laloknam S (2005) Nitrate uptake in the halotolerant cyanobacterium Aphanothene halophytica is energy-dependent driven by ${\Delta}pH$. J Biochem Mol Biol 38, 468-473.   DOI   ScienceOn
10 Kang BG, Jeong IM, Kim JJ, Hong SD, and Min KB (1997) Chemical characteristics of plastic film house soils in Chungbuk area. Korean J Soil Sci Fert 30, 265-271.
11 Kang BG, Jeong IM, Min KB, and Kim JJ (1996) Effect of salt accumulation on the germination and growth of lettuce (Lactuca sativa, L.) Korean J Soil Sci Fert 29, 360-364.
12 Kang SS and Hong SD (2004) Estimation of optimum application rate of nitrogen fertilizer based on soil nitrate concentration for tomato cultivation in plastic film house. Korean J Soil Sci Fert 37, 74-82.
13 Kasprow RP, Lange AJ, and Kirwan DJ (1998) Correlation of fermentation yield with yeast extract composition as characterized by near-infrared spectroscopy. Biotechnol Prog 14, 318-325.   DOI   ScienceOn
14 Mori M, Amato M, Di Mola I, Caputo R, Quaglietta CF, and Di Tommaso T (2008) Productive behaviour of cherry-type tomato irrigated with saline water in relation to nitrogen fertilization. Eur J Agr 29, 135-143.   DOI   ScienceOn
15 Kim ST, Choi TG, Wang HS, and Kim YK (2009) Nitrate removal mediated by soil microorganism, Enterobacter sp. GG0461. J Gen Appl Microbiol 55, 75-79.   DOI   ScienceOn
16 Lee GJ, Kang BK, Kim HJ, Park SK, and Min KB (2001) Effect of nitrogen fertilizers on soil pH, EC, $NO_3-N$ and lettuce (Lactuca sativa L.) growth. Korean J Soil Sci Fert 34, 122-128.
17 McIsaac G (2003) Surface water: Pollution by nitrogen fertilizers. In Encyclopedia of Water Science, (2nd ed.), pp. 950−955, University of Illinois, Urbana, IL.
18 Mori M, Di Mola I, and Quaglietta CF (2011) Salt stress and transplant time in snap bean: Growth and productive behaviour. Int J Plant Prod 5, 49-64.
19 Oztukr A, Unlukara A, Ipek A, and Gurbuz B (2004) Effects of salt stress and water deficit on plant growth and essential oil content of lemon balm (Melissa officinalis L.). Pakistan J Bot 36, 787-792.
20 Steenhoudt O, Ping Z, Broek AV, and Vanderleyden J (2001) A spontaneous chlorate-resistant mutant of Azospirillum brasilense Sp245 displays defects in nitrate reduction and plant root colonization. Biol Fertil Soils 33, 317-322.   DOI   ScienceOn
21 Zayed G and Winter J (1998) Removal of organic pollutants and of nitrate from wastewater from the dairy industry by denitrification. Appl Microbiol Biotechnol 49, 469-474.   DOI   ScienceOn
22 Vilchez C, Garbayo I, Markvicheva E, Galvan F, and Leon R (2001) Studies on the suitability of alginate-entrapped Chlamydomonas reinhardtii cells for sustaining nitrate consumption process. Bioresour Technol 78, 55-61.   DOI   ScienceOn
23 Wang HS, Han MW, and Kim YK (2010) Chlorate-induced inhibition of nitrate uptake mediated by Enterobacter amnigenus GG0461. J Korean Soc Appl Biol Chem 53, 164-169.   DOI