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http://dx.doi.org/10.3839/jabc.2011.002

Optimization of Medium Components for the Production of Crude Biosurfactant by Bacillus subtilis JK-1  

Joo, Myeong-Hoon (Graduate School of Molecular and Biomedical Technology, Inje University)
Kim, Ji-Yeon (College of General Education, Inje University)
Publication Information
Journal of Applied Biological Chemistry / v.54, no.1, 2011 , pp. 7-14 More about this Journal
Abstract
Bacillus subtilis JK-1 showed degradation activity against crude oil, gasoline, kerosene, and light oil, and this strain was used as a crude biosurfactant producing microorganism in this study. To optimize the culture medium for production of crude biosurfactant, the influences of various carbon, nitrogen and mineral sources were assessed. The highest biosurfactant production by B. subtilis JK-1 was observed after 96 h cultivation, containing 1.0% (w/v) soluble starch as a carbon source and 0.5% (w/v) skim milk as a nitrogen source, and carbon to nitrogen concentraion (C/N) ratio was 2.0. For the biosurfactant production 0.1% (w/v) of $KNO_3$ was the most effective mineral source. Comparison of biosurfactant production indicates that B. subtilis JK-1 produces more biosurfactant in the optimum medium established in this study than LB and TSB. Under the optimum medium, the surface tension of culture broth of B. subtilis JK-1 was decreased from 47.3 dyne/cm to 24.0 dyne/cm after cultivation of 48 h.
Keywords
Bacillus subtilis JK-1; biosurfactant production; C/N ratio; carbon source; medium optimization; mineral source; nitrogen source; surface tension;
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