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http://dx.doi.org/10.3839/jabc.2010.014

Sterols from the Seed of Cowpea (Vigna sinensis K.)  

Cui, En-Ji (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University)
Park, Hee-Jung (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University)
Wu, Qian (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University)
Chung, In-Sik (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University)
Kim, Ji-Young (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University)
Baek, Nam-In (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University)
Publication Information
Journal of Applied Biological Chemistry / v.53, no.2, 2010 , pp. 77-81 More about this Journal
Abstract
The seed of cowpea (Vigna sinensis K.) was extracted with 80% aqueous methanol (MeOH). And the concentrated extract was partitioned with ethyl acetate (EtOAc), n-butanol (n-BuOH) and $H_2O$, successively. The repeated silica gel and octadecyl silica gel (ODS) column chromatographic separations for the EtOAc and n-BuOH fractions led to isolation of four sterols. And the chemical structures of the compounds were determined as a mixture of stigmasterol and $\beta$-sitosterol with the ratio of 4 to 3 (1), 7-ketositosterol (2), and stigmasterol 3-O-$\beta$D-glucopyranoside (3) from the interpretation of spectroscopic data including nuclear magnetic resonance (NMR) spectrum metric, mass (MS) spectrum metric and infrared (IR) spectroscope. This study reports the first isolation of $\beta$-sitosterol, 7-ketositosterol, and stigmasterol 3-O-$\beta$-D-glucopyranoside from the seed of Vigna sinensis K. In addition, compound 2, 7-ketositosterol, is rarely occurred in natural source including plant.
Keywords
7-ketositosterol; $\beta$-sitosterol; cowpea; stigmasterol; stigmasterol; 3-O-$\beta$-D-glucopyranoside; Vigna sinensis;
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Times Cited By KSCI : 2  (Citation Analysis)
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