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http://dx.doi.org/10.3839/jabc.2010.021

Production of Bacteriophytochrome Specific Antibodies of Deinococcus radiodurans  

Kim, Tae-Lim (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University)
Hahn, Tae-Ryong (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University)
Bhoo, Seong-Hee (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University)
Publication Information
Journal of Applied Biological Chemistry / v.53, no.2, 2010 , pp. 112-115 More about this Journal
Abstract
To analyze the surface properties of bacteriophytochrome (BphP), five (2B8, 2C11, 3B2, 3D2, 3H7) anti-BphP monoclonal antibodies were produced by using full-length of BphP of Deinococcus radiodurnas. 2B8 and 2C11 preferentially recognized the epitopes at N-terminal region of BphP, whereas 3B2, 3D2 and 3H7 showed preferential affinities to the epitopes of C-terminal region of BphP.
Keywords
bacteriophytochrome; deinococcus radiodurnas; monoclonal antibody;
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