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http://dx.doi.org/10.3839/jabc.2010.019

Effect of Agarase Signal Peptide from Agarivorans albus YKW-34 on Protein Secretion in Escherichia coli  

Lee, Joo-Young (Faculty of Marine Bioscience and Technology, Division of Marine Bioscience Engineering, Kangnung-Wonju National University)
Song, Dae-Geun (Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute)
Son, Jin-Ki (Faculty of Marine Bioscience and Technology, Division of Marine Bioscience Engineering, Kangnung-Wonju National University)
Pan, Cheol-Ho (Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute)
Publication Information
Journal of Applied Biological Chemistry / v.53, no.2, 2010 , pp. 105-107 More about this Journal
Abstract
To overcome the limitation of E. coli expression system such as inclusion body formation and disulfide bond failure, we tried to express the heterologous protein as a secreted form. We adopted agarase signal peptide (ASP; 23 amino acid residues) from Agarivorans albus YKW-34 which is one of marine bacteia. When we used ASP to express $\beta$-agarase, about 42% activity was detected in media.
Keywords
Agarivorans albus; Escherichia coli; recombinant protein; secretion; signal peptide;
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Times Cited By KSCI : 2  (Citation Analysis)
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