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http://dx.doi.org/10.3839/jabc.2009.032

ACAT (Acyl-CoA:cholesterol Acyltransferase) Inhibitory Effect and Quantification of Pyranocurmarin in Different Parts of Angelica gigas Nakai  

Kim, Geum-Soog (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA)
Park, Chun-Geun (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA)
Jeong, Tae-Sook (Korea Research Institute of Bioscience & Biotechnology)
Cha, Seon-Woo (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA)
Baek, Nam-In (Graduate School of Biotechnology, Kyung Hee University)
Song, Kyung-Sik (College of Agriculture and Life Sciences, Kyungpook National University)
Publication Information
Journal of Applied Biological Chemistry / v.52, no.4, 2009 , pp. 187-194 More about this Journal
Abstract
Two pyranocoumarin constituents have been isolated from Angelica gigas and were identified as decursinol angelate (1) and decursin (2) by means of NMR analysis, respectively. Human acyl-CoA:cholesterol acyltransferase (hACAT) inhibitory activity of decursinol angelate (1) and decursin (2) was evaluated. Decursin (2) showed significantly inhibitory activity against hACAT1 and hACAT2 with $IC_{50}$ value of 137 and $168\;{\mu}M$, respectively, whereas decursinol angelate (1) exhibited weak ACAT inhibitory activity. These results suggested that decusin from A. gigas might be effective for the prevention and the treatment of hypercholesterolemia or atherosclerosis by inhibitory effect on hACAT. The contents of decursinol angelate (1) and decursin (2) were analyzed in various parts of A. gigas including flower, seed, leaf and root using LC/MS/MS (ESI, positive ion mode, MRM mode). The content of decursinol angelate was increased in order of flower, seed, leaf, and root and decursin content was increased in order of flower, seed, leaf, and root. It was expected that unused parts including leaf and flower of A. gigas might be useful as new functional sources by their high contents of decursin and decursinol angelate.
Keywords
ACAT (Acyl-CoA:cholesterol acyltransferase); Angelica gigas; decursin; decursinol angelate; LC/MS/MS;
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Times Cited By KSCI : 9  (Citation Analysis)
Times Cited By SCOPUS : 2
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