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http://dx.doi.org/10.3839/jabc.2009.026

Isolation of Lipase Producing Bacillus subtilis and Some Characteristics of the Enzyme  

Jo, Ji-Won (Graduate School of Molecular & Biomedical Technology, Inje University)
Hur, Sung-Ho (Department of Clinical Laboratory Science, Dong-eui Institute of Technology)
Han, Yong-Soo (Department of Cosmetology, Gwangyang Health College)
Kim, Ji-Yeon (Graduate School of Molecular & Biomedical Technology, College of General Education, Inje University)
Publication Information
Journal of Applied Biological Chemistry / v.52, no.3, 2009 , pp. 151-156 More about this Journal
Abstract
As part of an investigation to identify microorganisms that are biotechnologically interesting for industrial application, we isolated a bacterial strain from Chungkookjang that produces extracellular neutral lipase. In addition, the crude enzyme was characterized. This isolated strain, designated as JKA-3 was identified as Bacillus subtilis JKA-3 based on morphological, physiological and biochemical characteristics, as well as phylogenetic analysis using 16S rRNA gene sequence. The cells were rod-shaped and $0.6-0.8{\times}2.0-2.3\;{\mu}m$ in size. Optimal growth conditions were $35-40^{\circ}C$ and pH 6.0-8.0. The isolate was able to grow in up to 0-10.0% (w/v) NaCl. Optimal activity conditions of the crude lipase fraction of B. subtilis JKA-3 were pH of 7.0 at $35^{\circ}C$. This enzyme was stable in the pH ranging 6.0-8.0.
Keywords
B. subtilis JKA-3; characterization; enzyme production; identification; neutral lipase;
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