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http://dx.doi.org/10.3839/jabc.2009.004

Comparison Study of White Ginseng, Red Ginseng, and fermented Red Ginseng on the Protective Effect of LPS-induced Inflammation in RAW 264.7 Cells  

Hyun, Mee-Sun (Professional Graduate School of Oriental Medicine, Wonkwang University)
Hur, Jung-Mu (Chong Kun Dang Healthcare Crop. Research Center)
Shin, Yong-Seo (Chong Kun Dang Healthcare Crop. Research Center)
Song, Bong-Joon (Chong Kun Dang Healthcare Crop. Research Center)
Mun, Yeun-Ja (Professional Graduate School of Oriental Medicine, Wonkwang University)
Woo, Won-Hong (Professional Graduate School of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Applied Biological Chemistry / v.52, no.1, 2009 , pp. 21-27 More about this Journal
Abstract
Red ginseng (RG) and fermented red ginseng (FRG) are produced from ginseng (GS) via certain biological processes. The main difference between three ginsengs is the composition of ginsenosides known as major metabolites having several biological activities. The concentration of the metabolites has been known to be dependent on the methods which make RG and FRG In this study, we investigated the effects of WG, RG and FRG on the productions of inflammatory proteins (NF-${\kappa}B$, iNOS, COX-2) and cytokines (TNF-$\alpha$, INF-$\gamma$) in LPS-stimulated RAW 264.7 cells. The levels of NO production and iNOS expression were suppressed by the treatment of white ginseng (WG), RG and FRG in LPS-stimulated cells. Also, the production of TNF-$\alpha$ and INF-$\gamma$ was decreased in the cells by all of them. It was indicated that the inhibition of NF-${\kappa}B$ activation in LPS-stimulated cells treated with three kinds of ginsengs was resulted from the suppression of the level of COX-2 expression and the phosphorylation of IkB by LPS. The present study indicated that RC showed the best biological activity among them and FRG was better than WG. The better activity of RG on the inhibition of NO production is considered to be caused by the difference of ginsenoside composition produced during their preparations. In order to elucidate the mechanism, animal test should be performed with three ginsengs.
Keywords
cytokine; fermented red ginseng; inflammation; red ginseng; white ginseng;
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1 Brown KL, Cosseau C, Gardy lL, and Hancock RE (2007) Complexities of targeting innate immunity to treat infection. Trends Immunol 28, 260-266   DOI   ScienceOn
2 Chen F, Castranova V, Shi X, and Demers LM (1999) New insights into the role of nuclear factor-kappaB, a ubiquitous transcription factor in the initiation of diseases. Clin Chem 45, 7-17   PUBMED   ScienceOn
3 Jang SK, Kim JH, Chung YS, Ahn DC, Kang MJ, Lee DO, and Kim SH (1994) An experimental study on the effect of Immunopotential and the Anticancer Effect of Red Ginseng Extract. J Ginseng Res 18, 151-159   과학기술학회마을
4 Kaltschmidt B, Spama T, and Kaltschmidt C (1999) Activation of NF -kappa B by reactive oxygen intermediates in the nervous system. Antioxid Redox Signal 1, 129-144   DOI   ScienceOn
5 Karin M and Ben-Neriah Y (2000) Phosphorylation meets ubiquitination: the control of NF-'kappa'B activity. Annu Rev Immunol 18, 621-663   DOI   ScienceOn
6 Scott MG and Hancock RE (2000) Cationic antimicrobial peptides and their multifunctional role in the immune system. Crit Rev Immunol 20, 407-431   PUBMED   ScienceOn
7 Feldmann M, Brennan FM, and Maini RN (1996) Role of cytokines in rheumatoid arthritis. Annu Rev Immunol 14, 397-440   DOI   ScienceOn
8 Iontcheva I, Amar S, Zawawi KH, Kantarci A, and Van Dyke TE (2004) Role for moesin in lipopolysacchruide-stimulated signal transduction. Infect Immun 72, 2312-2320   DOI   ScienceOn
9 Choi KH, Kwak YS, Rhee MH, Hwang MS, Kim SC, Park CK, Han GH, and Song KB (2008) Effects of pH and high temperature treatment on the changes of major ginsenosides composition in korean red ginseng water extract. J Ginseng Res 32, 127-134   DOI   ScienceOn
10 Mu MM, Chakravortty D, Sugiyama T, Koide N, Takahashi K, Mori I, Yoshida T, and Yokochi T (2001) The inhibitory action of quercetin on lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophage cells. J Endotoxin Res 7, 431-438   PUBMED   ScienceOn
11 Baldwin AS Jr (1996) The NF-kappa B and I kappa B proteins: new discoveries and insights. Annu Rev Immunol 14, 649-683   DOI   PUBMED   ScienceOn
12 Lazarov S, Balutsov M, and lanev E (2000) The role of bacterial endotoxins, receptors and cytokines in the pathogenesis of septic (endotoxin) shock. Vutr Boles 32, 33-40   PUBMED
13 Seybold VS, Jia YP, and Abrahams LG (2003) Cyclo-oxygenase-2 contributes to central sensitization in rats with peripheral inflammation. Pain 105, 47-55   DOI   ScienceOn
14 Cho JY, Kim AR, Yoo ES, Baik KU, and Park MH (2002) Ginsenosides from Panax ginseng differentially regulate lymphocyte proliferation. Planta Med 68, 497-500   DOI   ScienceOn
15 Surh YJ, Chun KS, Cha HH, Han SS, Keum YS, Park KK, and Lee SS (2001) Molecular mechanisms underlying chemopreventive activities of anti-inflammatory phytochemicals: down-regulation of COX-2 and iNOS through suppression of NF-kappa B activation. Mutat Res 480-481, 243-268   DOI   PUBMED   ScienceOn
16 Axtelle T and Pribble J (2001) ICI4, a CDl4 specific monoclonal antibody, is a potential treatment for patients with severe sepsis. J Endotoxin Res 7, 310-314   PUBMED   ScienceOn
17 Chow JC, Young DW, Golenbock DT, Christ WJ, and Gusovsky F (1999) Toll-like receptor-4 mediates lipopolysaccharide-induced signal transduction. J BioI Chem 274, 10689-10692   DOI   ScienceOn
18 Straus OS, Pascual G, Li M, Welch JS, Ricote M, Hsiang CH, Sengchanthalangsy LL, Ghosh G, and Glass CK (2000) 5- deoxy-delta 12,14-prostaglandin J2 inhibits mUltiple steps in the NF-kappa B signaling pathway. Proc Natl Acad Sci USA 97, 4844-4849   DOI   ScienceOn
19 Baeuerle PA (1998) I kappa B - NF-kappa B structures: at the interface of inflammation control. Cell 95, 729-731   DOI   PUBMED   ScienceOn
20 Stokes KY, Cooper D, Tailor A, and Granger DN (2002) Hypercholesterolemia promotes inflammation and microvascular dysfunction: role of nitric oxide and superoxide. Free Radical BioI Med 33, 1026-1036   DOI   ScienceOn
21 Lee EJ, Ko E, Lee J, Rho S, Ko S, Shin MK, Min 81, Hong MC, Kim SY, and Bae H (2004) Ginsenoside Rgl enhances CD4(+) T-cell activities and modulates ThIlTh2 differentiation. Int J Immunopharmaco 4, 235-244   DOI   ScienceOn
22 Palmer RM and Eveson JW (1987) Chronic sialadenitis. An immunocytochemical study in humans. Virchows Arch A Pathol Anat Histopathol 412, 73-78   DOI   PUBMED
23 Vane JR, Bakhle YS, and Botting RM (1998) Cyclooxygenases 1 and 2. Annu Rev Pharmacol Toxicol 38, 97-120   DOI   ScienceOn
24 Sun JL, Hu YL, Wang DY, Zhang BK, and Liu JG (2006) Immunologic enhancement of compound Chinese herbal medicinal ingredients and their efficacy comparison with compound Chinese herbal medicines. Vaccine 24, 2343-2348   DOI   ScienceOn
25 Means TK, Golenbock DT, and Fenton MJ (2000) The biology of Toll-like receptors. Cytokine Growth Factor Rev 11, 219-232   DOI   ScienceOn
26 Posadas I, Terencio MC, Guilln I, Ferrndiz ML, Coloma J, PayM, and Alcaraz MJ (2000) Co-regulation between cyclo-oxygenase- 2 and inducible nitric oxide synthase expression in the timecourse of murine inflammation. Naunyn-Schmiedebergs Arch Pharmacol 361, 98-106   DOI   ScienceOn
27 Lee HY and Lee HS (1998) Stimulatory effect of korean redginseng extract on the proliferation and cellular activity of lymphocytes. J Ginseng Res 22, 60-65
28 Lee WM, Kim SD, Kim KS, Song YB, Kwak YS, Cho IY, Park HJ, Oh JW, and Rhee MH (2006) Protopanaxadiol modulates LPS-induced inflammatory activity in murine macrophage RAW264.7 cells. J Ginseng Res 30, 181-187   DOI   ScienceOn
29 Yun HY, Dawson VL, and Dawson TM (1996) Neurobiology of nitric oxide. Crit Rev Neurobiol 10, 291-316   PUBMED   ScienceOn
30 Moncada S, Palmer RM, and Higgs EA (1991) Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacol Rev 43, 109-142   PUBMED   ScienceOn
31 Needleman P and Isakson PC (1997) The discovery and function of COX-2. J Rheumatol Suppl 49, 6-8   PUBMED
32 Gomez PF, Pillinger MH, Attur M, Marjanovic N, Dave M, Park J, Bingham CO 3rd, Al-Mussawir H, and Abramson SB (2005) Resolution of inflammation: prostaglandin E2 dissociates nuclear trafficking of individual NF-kappa B subunits (p65, p50) in stimulated rheumatoid synovial fibroblasts. J Immunol 175, 6924-6930   PUBMED   ScienceOn
33 Ryu JH, Ahn H, Kim JY, and Kim YK (2003) Inhibitory activity of plant extracts on nitric oxide synthesis in LPS-activated macrophages. Phytother Res 17, 485-489   DOI   ScienceOn
34 Kong BM, Park MJ, Min JW, Kim HB, Kim SH, Kim SY, and Yang DC (2008) Physico-chemical characteristics of White, Fermented and Red Ginseng Extracts. J Ginseng Res 32, 238-243   DOI   ScienceOn