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http://dx.doi.org/10.3839/jabc.2009.001

Qualitative PCR Detection of Stack Gene GM Rice (LS28 X Cry1Ac) Developed in Korea  

Shin, Kong-Sik (National Academy of Agricultural Science, RDA)
Park, Jong-Hyun (National Academy of Agricultural Science, RDA)
Lee, Jin-Hyoung (National Academy of Agricultural Science, RDA)
Lee, Si-Myung (National Academy of Agricultural Science, RDA)
Woo, Hee-Jong (National Academy of Agricultural Science, RDA)
Lim, Sun-Hyung (National Academy of Agricultural Science, RDA)
Kim, Hae-Yeong (Department of Food Science and Biotechnology, Kyung Hee University)
Suh, Seok-Cheol (National Academy of Agricultural Science, RDA)
Kweon, Soon-Jong (National Academy of Agricultural Science, RDA)
Publication Information
Journal of Applied Biological Chemistry / v.52, no.1, 2009 , pp. 1-7 More about this Journal
Abstract
For the development of qualitative PCR detection method of genetically modified (CM) rice, rice species-specific gene, OsCc-1 (rice cytochrome c gene), was selected as suitable far use as an endogenous gene in rice. The primer pair OsCytC-5'/3'with 111 bp amplicon was used for PCR amplification of the rice endogenous gene, OsCc-1 and no amplified product was observed from 8 different crops as templates. Qualitative PCR method was carried out with stack traits of L528$\times$CryIAc1 GM rice developed in Korea. For the qualitative PCRs, some primer pairs were designed with a construct-specific and event-specific type based on T-DNA and junction sequences of T-DNA in GM rice. Actck-5'/3' amplifying between actin promoter and OsCK1 gene introduced in LS28 gave rise to an amplicon 306 bp; also, CrLB-5'/3' from CryIAcl and CKRB-5'/3'amplifying the junction region of T-DNA and genome sequence from LS28 as event-specific primers gave rise to an amplicon 142 bp and 91 bp, respectively. These primer pairs for the detection of event-specific targets not produced PCR amplicons on non-CM rice and various crops in contrast to event lines. Therefore, in this study we verified that event-specific primers were effective to specifically detect stack trait lines and demonstrated that this method presented could be provided with the detection-method data for risk assessment analysis of GM rice to be commercialized.
Keywords
event-specific primer; GM rice; qualitative PCR; stack traits;
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Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By SCOPUS : 1
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