Browse > Article

Radical Scavenging Activity and Content of Cynarin (1,3-dicaffeoylquinic acid) in Artichoke (Cynara scolymus L.)  

Jun, Neung-Jae (National Institute of Subtropical Agriculture, R.D.A.)
Jang, Ki-Chang (National Institute of Subtropical Agriculture, R.D.A.)
Kim, Seong-Cheol (National Institute of Subtropical Agriculture, R.D.A.)
Moon, Doo-Young (National Institute of Subtropical Agriculture, R.D.A.)
Seong, Ki-Cheol (National Institute of Subtropical Agriculture, R.D.A.)
Kang, Kyung-Hee (National Institute of Subtropical Agriculture, R.D.A.)
Tandang, Leoncia (National Institute of Subtropical Agriculture, R.D.A.)
Kim, Phil-Hoon (Aria Jeju, B.I.C., Cheju Halla College)
Cho, So-Mi K. (Faculty of Biotechnology, College of Applied Life Sciences, Cheju National University)
Park, Ki-Hun (Division of Applied Life Science (BK21 Program), Environmental Biotechnology National Core Research Center, Research Institute of Life Science, Gyeongsang National University)
Publication Information
Journal of Applied Biological Chemistry / v.50, no.4, 2007 , pp. 244-248 More about this Journal
Abstract
The contents of total phenol and total flavonoid of artichoke (Cynara scolymus L.) were measured. The antioxidant activity of the artichoke was evaluated based on its potential as a scavenging the ABTS radical. These results showed the antioxidant activity of artichoke has a close relationship with the total flavonoid content. The compound showing antioxidant activity was isolated from the artichoke by repeated column chromatography and recrystallization. Based on the spectrometric studies, the compound was identified as 1,3-dicaffeoylquinic acid, known as cynarin. The content of cynarin from heads and leafs of the artichoke determined by $C_{18}$ reversed phase HPLC (high-performance liquid chromatography) coupled with photodiode array detector was 10.15 and 0.67 mg/g, respectively. This compound showed potent antioxidant activities against DPPH and ABTS radicals ($EC_{50}$ = 14.09 and 28.85 ${\mu}M$, respectively).
Keywords
antioxidant activity; artichoke; Cynara scolymus L.; cynarin; total flavonoid; total phenol;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Dranik LI, Dolganenko LG, Slapke J, and Thoma N (1996) Chemical composition and medical usage of Cynara scolymus L. Rastit Resur 32, 98-104
2 Garcia SM, Firpo IT, Anido FSL, and Cointry, EL (1999) Application of gibberellic acid in globe artichoke. Pesqui Agropecu Bras 34, 789-793   DOI
3 Hatano T, Kagawa H, Yasuhara T, and Okuda T (1988) Two new flavonoids and other constituents in licorice root: Their relative astringency and radical scavenging effects. Chem Pharm Bull 36, 1090-2097
4 Kocer G and Eser B (1999) Assessment of the effects of rooted offshoot properties and GA3 applications on the yield of the globe artichoke. Turk J Agric Forest 23, 325-332
5 Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, and Rice-Evans C (1999) Antioxidant activity applying improved ABTS radical cation decolorization assay. Free Radical Bio Med 26, 1231-1237   DOI   ScienceOn
6 Wagenbreth D (1996) Evaluation of artichoke cultivars for growing and pharmaceutical use. Beitr Zuchtungsforsch 2, 400-403
7 Schram K, Miketova P, Slanina J, Humpa O, and Taborska E (2004) Mass spectrometry of 1,3- and 1,5-dicaffeoylquinic acids. J Mass Spectrom 39, 384-395   DOI   ScienceOn
8 De Malach JG, Sachs M, and Rotem R (1976) Timing and Optimal Concentration of Gibberellic Acid Treatments for Forcing Yield of Globe Artichoke (Cynara scolymus L.) Edizioni Minerva Medica, Turin, Italy, pp. 633-642
9 Nichiforescu EA (1970) Composition of caffeoylquinic acid derivatives of artichoke (Cynara scolymus L.). Plant Med Phytother 4, 56-62
10 Schrader WL (1994) Growth regulator gives earlier harvest in artichokes. Calif Agric 48, 29-32
11 Garcia SM, Firpo IT, Lopez-Anido FS, and Cointry EL, (1998) Influence of cultural techniques on the profitability of globe artichoke (Cynara scolymus L.) in Argentina. Avances en Horticultura 3, 43-48
12 Adzet T and Puigmacia M (1985) High-performance liquid chromatography of caffeoylquinic acid derivatives of Cynara scolymus L. leaves. J Chromatogr A 348, 447- 452   DOI   ScienceOn
13 Choi YM, Ku JB, Chang HB, and Lee JS (2005) Antioxidant activities and total phenolics of ethanol extracts from several edible mushrooms produced in Korea. Food Sci Biotechnol 14, 700-703
14 Foury C (1977) Trials on gibberellic acid application to a spring crop of globe artichoke (Cynara scolymus) cv. Blanc Hyerois. Annales de L'Amelioration des Plantes 27, 411-426
15 Wang MF, Simon JE, Aviles IF, He K, Zheng QY, and Tadmor Y (2003) Analysis of antioxidative phenolic compounds in antichoke (Cynara scolymus L.). J Agr Food Chem 51, 601-608   DOI   ScienceOn
16 Zaki EM, Abed T.A, Gabal MR, and El-Abagy MM, (1991) Effect of some growth regulators on growth, yield and phenolic substances of artichoke plants Cynara scolymus L. Ann Agric Sci Moshtohor 26, 1939-1957
17 Horman I, Badoud R, and Ammann W (1984) Food-related application of one- and two-dimensional high-resolution proton nuclear magnetic resonance: structure and conformation of cynarin. J Agr Food Chem 32, 538-540   DOI
18 Gebhardt R (1997) Antioxidative and protective properties of extracts from leaves of the artichoke (Cynara scolymus L.) against hydroperoxide-induced oxidative stress in cultured rat hepatocytes. Toxicol Appl pharm 144, 279-286   DOI   ScienceOn
19 Jo Jo and Jung IC. (2000) Changes in carotenoid contents of several green-yellow vegetables by blanching . Korean J Soc Food Sci 16, 17-21
20 Price MP and Buttler LG. (1977). Rapidvisual estimation and spectrophotometic determination of tannin content of Sorghum grain. J Agr Food Chem 25, 1268-1273   DOI
21 Miguel A, Maroto JV, Iranzo B, and Loez-Galarza S (1997) Gibberellic acid in artichoke. Horticultura, Revista de Hortalizas, Flores y Plantas Ornamentales 120, 111-113