Browse > Article

Enhanced Production of L-Aspartate ${\beta}-Decarboxylase$ by Nitrogen Source in Pseudomonas dacunhae  

Kim, Dong-Chung (Department of Food Science, Suncheon First College)
Lee, Sung-Dong (Department of Food and Nutrition, College of Health Science, Korea University)
In, Man-Jin (Department of Human Nutrition and Food Science, Chungwoon University)
Publication Information
Journal of Applied Biological Chemistry / v.49, no.3, 2006 , pp. 106-109 More about this Journal
Abstract
Improvement of L-asparate ${\beta}-decarboxylase$ production from Pseudomonas dacunhae ATCC 21192 was attempted by optimizing fermentation conditions. Optimum carbon and nitrogen sources for cell growth and enzyme production were determined. L-Glutamate (2%) was the most suitable carbon source, and D-glucose, D-glycerol and fumarate repressed enzyme production. Yeast extract (2%) was the most effective as nitrogen source. A slight change of pH to 6.5 from medium pH resulted in a meaningful increase in the production of enzyme. The production of the enzyme was highly improved by using 2% yeast extract and 2% L-glutamate in culture media. Maximum L-asparate ${\beta}-decarboxylase$ activity reached up to over 24 U/mL-broth by 15 h flask fermentation.
Keywords
Pseudomonas dacunhae; L-asparate ${\beta}-decarboxylase$; L-glutamate; yeast extract;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Fusee, M. C. and Weber, J. E. (1984) Immobilization by polyurethane of Pseudomonas dacunhae containing L-aspartate $\beta$-decarboxylase activity and application to L-alanine production. Appl. Environ. Microbiol. 48, 694-698
2 Calk, G., Vural, H. and Ozdamar, T. H. (1997) Bioprocess parameters and oxygen transfer effects in the growth of Pseudomonas dacunhae for L-alanine production. Chem. Eng. J. 65, 109-116   DOI   ScienceOn
3 Joseph, M. H. and Davies, P. (1983) Electrochemical activity of o-phthalaldehyde-mercaptoethanol derivatives of amino acids. Application to high-performance liquid chromatographic determination of amino acids in plasma and other biological materials. J. Chromatogr. 277, 125-136   DOI   ScienceOn
4 Varela, H., Ferrari, M. D., Belobradjic, L., Weyrauch, R. and Loperena, M. L. (1996) Effect of medium composition on the production by a new Bacillus subtilis isolate of protease with promising unhairing activity. World J. Microbiol. Biotechnol. 12, 643-645   DOI   ScienceOn
5 Albers, E., Larsson, C., Liden, G., Niklasson, C. and Gustafsson, L. (1996) Influence of the nitrogen source on Saccharomyces cerevisiae anaerobic growth and product formation. Appl. Environ. Microbiol. 62, 3187-3195
6 Calk, G., Savasc, H., Calyk, P. and Ozdamar, T. H. (1999) Growth and k-carrageenan immobilization of Pseudomonas dacunhae cells for L-alanine production. Enzyme Microb. Technol. 24, 67-74   DOI   ScienceOn
7 Shibatani, T., Kakimoto, T. and Chibata, I. (1979) Stimulation of L-aspartate $\beta$-decarboxylase formation by Lglutamine in Pseudomonas dacunhae and improved production of L-alanine. Appl. Environ. Microbiol. 38, 359-364
8 Ishizaki-Koizumi, S., Sonaka, I., Fujitani, S. and Nishiguchi, S. (2002) Mechanisms of the protective effect of L-alanine to D-galactosemia-induced hepatocellular injury: comparative studies of L-alanine and pyruvate. Biochem. Biophys. Res. Commun. 291, 738-743   DOI   ScienceOn
9 Yamamoto, K., Tosa, T. and Chibata, I. (1980) Continuous production of L-alanine using Pseudomonas dacunhae immobilized with carrageenan. Biotechnol. Bioeng. 22, 2045-2054   DOI
10 Burdick, B. A. and Schaeffer, J. R. (1988) Production of speciality chemicals using immobilized whole cells in spiral wound bioreactor: L-Aspartic acid and alanine production using cells immobilized in gelatine on nylon mesh. Biotechnol. Bioeng. 31, 390-395   DOI   ScienceOn
11 Chibata, I., Tosa, T. and Takamatsu, S. (1987) Continuous L-alanine production using two different immobilized microbial cell production. Methods Enzymol. 136, 472-479   DOI
12 Chibata, I., Kakimoto, T. and Kato, J. (1965) Enzymatic production of L-alanine by Pseudomonas dacunhae. Appl. Microbiol. 13, 638-645
13 Maezono, K., Kajiwara, K., Mawatari, K., Shinkai, A., Torii, K. and Maki, T. (1996) Alanine protects liver injury caused by D-galactosemia and $CCl_{4}$. Hepatology 24, 185- 191
14 Kim, D. C, Oh, N. S. and In, M. -J. (2004) Effect of carbon and nitrogen sources cell growth and halotolerant alkaline protease production in Halomonas marisflava isolated from salt-fermented food. Food Sci. Biotechnol. 13, 837- 840