[KSCI] Korea Science Citation Index Service

Functional Identification of Ginkgo biloba 1-Deoxy-D-xylulose 5-Phosphate Synthase (DXS) Gene by Using Escherichia coli Disruptants Defective in DXS Gene

Kim, Sang-Min (Program in Applied Life Chemistry, School of Agricultural Biotechnology, Seoul National University)
Kuzuyama, Tomohisa (Laboratory of Cell Biotechnology, Biotechnology Research Center, University of Tokyo)
Chang, Yung-Jin (Program in Applied Life Chemistry, School of Agricultural Biotechnology, Seoul National University)
Kim, Soo-Un (Program in Applied Life Chemistry, School of Agricultural Biotechnology, Seoul National University)

Publication Information

Journal of Applied Biological Chemistry / v.48, no.2, 2005 , pp. 101-104 More about this Journal

Abstract

DXS catalyzes the first step of MEP pathway. Escherichia coli disruptants defective in dxs were constructed by insertional mutation and characterized. Selected disruptant, DXM3, was auxotrophic for DX or ME. Putative class 1 DXS ORF from Ginkgo biloba was shown to rescue DXM3 grown without DX or ME supplementation. The putative ORF was thus confirmed as DXS1. The disruptant was demonstrated to be useful for DSX screening.

Keywords

1-Deoxy-D-xylulose 5-phosphate synthase; Ginkgo biloba; dxs disruptant;

Citations & Related Records

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