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http://dx.doi.org/10.4162/nrp.2021.15.6.673

Effects of in vitro vitamin D treatment on function of T cells and autophagy mechanisms in high-fat diet-induced obese mice  

Kang, Min Su (Department of Food and Nutrition, College of Human Ecology, Seoul National University)
Park, Chan Yoon (Department of Food & Nutrition, College of Health Science, The University of Suwon)
Lee, Ga Young (Department of Food and Nutrition, College of Human Ecology, Seoul National University)
Cho, Da Hye (Department of Food and Nutrition, College of Human Ecology, Seoul National University)
Kim, So Jeong (Department of Food and Nutrition, College of Human Ecology, Seoul National University)
Han, Sung Nim (Department of Food and Nutrition, College of Human Ecology, Seoul National University)
Publication Information
Nutrition Research and Practice / v.15, no.6, 2021 , pp. 673-685 More about this Journal
Abstract
BACKGROUND/OBJECTIVES: Obesity is associated with the impaired regulation of T cells characterized by increased numbers of Th1 and Th17 cells and the dysregulation of vitamin D metabolism. Both obesity and vitamin D have been reported to affect autophagy; however, a limited number of studies have investigated the effects of vitamin D on T cell autophagy in obese mice. Therefore, we aimed to determine whether in vitro treatment with vitamin D affects the proliferation, function, and autophagy of T cells from obese and control mice. MATERIALS/METHODS: Five-week-old male C57BL/6 mice were fed control or high-fat diets (10% or 45% kcal fat: CON or HFDs, respectively) for 12 weeks. Purified T cells were stimulated with anti-CD3 and anti-CD28 monoclonal antibodies and cultured with either 10 nM 1,25(OH)2D3 or 0.1% ethanol (vehicle control). The proliferative response; expression of CD25, Foxp3, RORγt, and autophagy-related proteins (LC3A/B, SQSTM1/P62, BECLIN-1, ATG12); and the production of interferon (IFN)-γ, interleukin (IL)-4, IL-17A, and IL-10 by T cells were measured. RESULTS: Compared with the CON group, T cell proliferation tended to be lower, and the production of IFN-γ was higher in the HFD group. IL-17A production was reduced by 1,25(OH)2D3 treatment in both groups. The LC3 II/I ratio was higher in the HFD group than the CON group, but P62 did not differ. We observed no effect of vitamin D treatment on T cell autophagy. CONCLUSIONS: Our findings suggest that diet-induced obesity may impair the function and inhibit autophagy of T cells, possibly leading to the dysregulation of T cell homeostasis, which may be behind the aggravation of inflammation commonly observed in obesity.
Keywords
Obesity; vitamin D; T lymphocytes; autophagy;
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