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http://dx.doi.org/10.4162/nrp.2015.9.1.3

Intestinal anti-inflammatory activity of Sasa quelpaertensis leaf extract by suppressing lipopolysaccharide-stimulated inflammatory mediators in intestinal epithelial Caco-2 cells co-cultured with RAW 264.7 macrophage cells  

Kim, Kyung-Mi (Department of Nutritional Science and Food Management, Ewha Womans University)
Kim, Yoo-Sun (Department of Nutritional Science and Food Management, Ewha Womans University)
Lim, Ji Ye (Department of Nutritional Science and Food Management, Ewha Womans University)
Min, Soo Jin (Department of Nutritional Science and Food Management, Ewha Womans University)
Ko, Hee-Chul (Jeju Sasa Industry Development Agency, Jeju National University)
Kim, Se-Jae (Department of Biology, Jeju National University)
Kim, Yuri (Department of Nutritional Science and Food Management, Ewha Womans University)
Publication Information
Nutrition Research and Practice / v.9, no.1, 2015 , pp. 3-10 More about this Journal
Abstract
BACKGROUND/OBJECTIVES: Inflammatory bowel disease (IBD), including Crohn's disease and ulcerative colitis, involves chronic inflammation of the gastrointestinal tract. Previously, Sasa quelpaertensis leaves have been shown to mediate anti-inflammation and anti-cancer effects, although it remains unclear whether Sasa leaves are able to attenuate inflammation-related intestinal diseases. Therefore, the aim of this study was to investigate the anti-inflammatory effects of Sasa quelpaertensis leaf extract (SQE) using an in vitro co-culture model of the intestinal epithelial environment. MATERIALS/METHODS: An in vitro co-culture system was established that consisted of intestinal epithelial Caco-2 cells and RAW 264.7 macrophages. Treatment with lipopolysaccharide (LPS) was used to induce inflammation. RESULTS: Treatment with SQE significantly suppressed the secretion of LPS-induced nitric oxide (NO), prostaglandin $E_2$ ($PGE_2$), IL-6, and IL-$1{\beta}$ in co-cultured RAW 264.7 macrophages. In addition, expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, and tumor necrosis factor (TNF)-${\alpha}$ were down-regulated in response to inhibition of $I{\kappa}B{\alpha}$ phosphorylation by SQE. Compared with two bioactive compounds that have previously been identified in SQE, tricin and P-coumaric acid, SQE exhibited the most effective anti-inflammatory properties. CONCLUSIONS: SQE exhibited intestinal anti-inflammatory activity by inhibiting various inflammatory mediators mediated through nuclear transcription factor kappa-B (NF-kB) activation. Thus, SQE has the potential to ameliorate inflammation-related diseases, including IBD, by limiting excessive production of pro-inflammatory mediators.
Keywords
Sasa quelpaertensis; co-culture model; anti-inflammation; pro-inflammatory mediators;
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