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http://dx.doi.org/10.4162/nrp.2013.7.6.481

Effects of d-${\alpha}$-tocopherol supplements on lipid metabolism in a high-fat diet-fed animal model  

Kim, Do Yeon (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University)
Kim, Jinkyung (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University)
Ham, Hye Jin (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University)
Choue, Ryowon (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University)
Publication Information
Nutrition Research and Practice / v.7, no.6, 2013 , pp. 481-487 More about this Journal
Abstract
High-fat diet up-regulates either insulin resistance or triglycerides, which is assumed to be related to the expression of peroxisome proliferator-activated receptor (PPAR)-${\alpha}$ and PPAR-${\gamma}$. The beneficial effects of vitamin E on insulin resistance are well known; however, it is not clear if vitamin E with a high-fat diet alters the expression of PPAR-${\alpha}$ and PPAR-${\gamma}$. We investigated the effects of d-${\alpha}$-tocopherol supplementation on insulin sensitivity, blood lipid profiles, lipid peroxidation, and the expression of PPAR-${\alpha}$ and PPAR-${\gamma}$ in a high-fat (HF) diet-fed male C57BL/6J model of insulin resistance. The animals were given a regular diet (CON; 10% fat), a HF diet containing 45% fat, or a HF diet plus d-${\alpha}$-tocopherol (HF-E) for a period of 20 weeks. The results showed that the HF diet induced insulin resistance and altered the lipid profile, specifically the triglyceride (TG) and total cholesterol (TC) levels (P < 0.05). In this animal model, supplementation with d-${\alpha}$-tocopherol improved insulin resistance as well as the serum levels of TG and very-low-density lipoprotein-cholesterol (VLDL-C) (P < 0.05). Moreover, the treatment decreased the levels of malondialdehyde (MDA) in the serum and liver while increasing hepatic PPAR-${\alpha}$ expression and decreasing PPAR-${\gamma}$ expression. In conclusion, the oral administration of d-${\alpha}$-tocopherol with a high-fat diet had positive effects on insulin resistance, lipid profiles, and oxidative stress through the expression of PPAR-${\alpha}$ and PPAR-${\gamma}$ in a high-fat diet-fed male mice.
Keywords
High fat diet; supplementation; d-${\alpha}$-tocopherol; insulin resistance;
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