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http://dx.doi.org/10.4162/nrp.2011.5.6.503

The anti-obesity effect of Lethariella cladonioides in 3T3-L1 cells and obese mice  

Sung, Ju-Hyun (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University)
Chon, Jeong-Woo (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University)
Lee, Mi-Ae (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University)
Park, Jin-Kyung (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University)
Woo, Jeong-Taek (Department of Endocrinology and Metabolism, Kyung Hee University School of Medicine)
Park, Yoo-Kyoung (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University)
Publication Information
Nutrition Research and Practice / v.5, no.6, 2011 , pp. 503-510 More about this Journal
Abstract
The aim of this study was to investigate whether a water extract of L. cladonioides (LC) has an anti-obesity effect in 3T3-L1 cells and obese mice. Treatment of differentiated 3T3-L1 adipocytes with LC caused a significant increase in glycerol release and reduced the protein expression of the adipogenic transcription factors, $PPAR{\gamma}$ and C/$EBP{\alpha}$. In an animal model, obese mice were artificially induced by a high fat diet for 10 weeks. Experimental groups were treated with LC (100 mg/kg/day) by gavage for the next 10 weeks. At the end of experiment, the body weight of the LC group mice was reduced by 14.2% compared to the high fat diet (HFD) group. The treatment also decreased liver (31.0%), epididymal (18.0%) and retroperitoneal (19.3%) adipose tissue, and kidney (6.7%) weights, respectively, compared with those of the HFD group. LC prevented diet-induced increases in the serum level of TC (22.6%), TG (11.6%), and glucose (35.0%), respectively, compared with the HFD group. However, the HDL-C level was higher in the LC group (26.1%) than the HFD group. The results of this study thus suggest that LC suppressed lipid accumulation and expression of adipogenic transcription factors, and increased the amount of glycerol release. LC also indicated an anti-obese and anti-hyperlipidemic effect.
Keywords
Lethariella cladonioides; obesity; 3T3-L1 cells; obese mice;
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1 Chun MR, Lee YJ, Kim KH, Kim YW, Park SY, Lee KM, Kim JY, Park YK. Differential effects of high-carbohydrate and high-fat diet composition on muscle insulin resistance in rats. J Korean Med Sci 2010;25:1053-9.   DOI   ScienceOn
2 Hursel R, Westerterp-Plantenga MS. Thermogenic ingredients and body weight regulation. Int J Obes (Lond) 2010;34:659-69.   DOI
3 Zheng G, Sayama K, Okubo T, Juneja LR, Oguni I. Anti-obesity effects of three major components of green tea, catechins, caffeine and theanine, in mice. In Vivo 2004;18:55-62.
4 Yun JW, Shin ES, Cho SY, Kim SH, Kim CW, Lee TR, Kim BH. The effects of BADGE and caffeine on the time-course response of adiponectin and lipid oxidative enzymes in high fat diet-fed C57BL/6J mice: correlation with reduced adiposity and steatosis. Exp Anim 2008;57:461-9.   DOI   ScienceOn
5 Murosaki S, Lee TR, Muroyama K, Shin ES, Cho SY, Yamamoto Y, Lee SJ. A combination of caffeine, arginine, soy isoflavones, and L-carnitine enhances both lipolysis and fatty acid oxidation in 3T3-L1 and HepG2 cells in vitro and in KK mice in vivo. J Nutr 2007;137:2252-7.   DOI
6 Mori S, Satou M, Kanazawa S, Yoshizuka N, Hase T, Tokimitsu I, Takema Y, Nishizawa Y, Yada T. Body fat mass reduction and up-regulation of uncoupling protein by novel lipolysispromoting plant extract. Int J Biol Sci 2009;5:311-8.
7 Cowherd RM, Lyle RE, McGehee RE Jr. Molecular regulation of adipocyte differentiation. Semin Cell Dev Biol 1999;10:3-10.   DOI   ScienceOn
8 Wolfram S, Wang Y, Thielecke F. Anti-obesity effects of green tea: from bedside to bench. Mol Nutr Food Res 2006;50:176-87.   DOI   ScienceOn
9 Basu A, Sanchez K, Leyva MJ, Wu M, Betts NM, Aston CE, Lyons TJ. Green tea supplementation affects body weight, lipids, and lipid peroxidation in obese subjects with metabolic syndrome. J Am Coll Nutr 2010;29:31-40.   DOI
10 Shehzad A, Ha T, Subhan F, Lee YS. New mechanisms and the anti-inflammatory role of curcumin in obesity and obesity-related metabolic diseases. Eur J Nutr 2011;50:151-61.   DOI   ScienceOn
11 Niu DL, Harada H, Wang LS, Zhang YJ, Yang CR. Chemotaxonomic study of the Lethariella cladonioides complex (lichenized Ascomycota, Parmeliaceae). Lichenologist 2011;43: 213-23.   DOI
12 Farmer SR. Regulation of PPARgamma activity during adipogenesis. Int J Obes (Lond) 2005;29 Suppl 1:S13-6.   DOI
13 Fajas L, Fruchart JC, Auwerx J. Transcriptional control of adipogenesis. Curr Opin Cell Biol 1998;10:165-73.   DOI   ScienceOn
14 Kinoshita K, Togawa T, Hiraishi A, Nakajima Y, Koyama K, Narui T, Wang LS, Takahashi K. Antioxidant activity of red pigments from the lichens Lethariella sernanderi, L. cashmeriana, and L. sinensis. J Nat Med 2010;64:85-8.   DOI   ScienceOn
15 Ramírez-Zacarías JL, Castro-Muñozledo F, Kuri-Harcuch W. Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil red O. Histochemistry 1992;97: 493-7.   DOI   ScienceOn
16 Folch J, Lees M, Sloane Stanley GH. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 1957;226:497-509.
17 Tzanavari T, Giannogonas P, Karalis KP. TNF-alpha and obesity. Curr Dir Autoimmun 2010;11:145-56.
18 Meier U, Gressner AM. Endocrine regulation of energy metabolism: review of pathobiochemical and clinical chemical aspects of leptin, ghrelin, adiponectin, and resistin. Clin Chem 2004;50: 1511-25.   DOI   ScienceOn
19 Moussalli C, Downs RW, May JM. Potentiation by glucose of lipolytic responsiveness of human adipocytes. Diabetes 1986;35: 759-63.   DOI   ScienceOn
20 Hauck M, Jürgens SR, Willenbruch K, Huneck S, Leuschner C. Dissociation and metal-binding characteristics of yellow lichen substances suggest a relationship with site preferences of lichens. Ann Bot 2009;103:13-22.   DOI
21 Park YS, Yoon Y, Ahn HS. Platycodon grandiflorum extract represses up-regulated adipocyte fatty acid binding protein triggered by a high fat feeding in obese rats. World J Gastroenterol 2007;13:3493-9.   DOI
22 Pittler MH, Schmidt K, Ernst E. Adverse events of herbal food supplements for body weight reduction: systematic review. Obes Rev 2005;6:93-111.   DOI   ScienceOn
23 Naik GH, Priyadarsini KI, Satav JG, Banavalikar MM, Sohoni DP, Biyani MK, Mohan H. Comparative antioxidant activity of individual herbal components used in Ayurvedic medicine. Phytochemistry 2003;63:97-104.   DOI   ScienceOn
24 Slanc P, Doljak B, Kreft S, Lunder M, Janes D, Strukelj B. Screening of selected food and medicinal plant extracts for pancreatic lipase inhibition. Phytother Res 2009;23:874-7.   DOI   ScienceOn
25 Saito M, Ueno M, Ogino S, Kubo K, Nagata J, Takeuchi M. High dose of Garcinia cambogia is effective in suppressing fat accumulation in developing male Zucker obese rats, but highly toxic to the testis. Food Chem Toxicol 2005;43:411-9.   DOI   ScienceOn
26 Qureshi K, Abrams GA. Metabolic liver disease of obesity and role of adipose tissue in the pathogenesis of nonalcoholic fatty liver disease. World J Gastroenterol 2007;13:3540-53.   DOI
27 Obermayer W. On the identity of lethariella sinensis wei & jiang, with new reports of tibetan lethariella species. Bibl Lichenol 2001;78:321-6.
28 Jiang B, Zhao QS, Yang H, Hou AJ, Lin ZW, Sun HD. Constituents from Lethariella cladonioides. Fitoterapia 2001;72: 832-3.   DOI   ScienceOn
29 Poulos SP, Dodson MV, Hausman GJ. Cell line models for differentiation: preadipocytes and adipocytes. Exp Biol Med (Maywood) 2010;235:1185-93.   DOI   ScienceOn
30 Lee WJ, Koh EH, Won JC, Kim MS, Park JY, Lee KU. Obesity: the role of hypothalamic AMP-activated protein kinase in body weight regulation. Int J Biochem Cell Biol 2005;37:2254-9.   DOI   ScienceOn
31 Kersten S. Mechanisms of nutritional and hormonal regulation of lipogenesis. EMBO Rep 2001;2:282-6.   DOI   ScienceOn
32 Frayn KN, Maycock PF. Skeletal muscle triacylglycerol in the rat: methods for sampling and measurement, and studies of biological variability. J Lipid Res 1980;21:139-44.
33 Jung RT, Shetty PS, James WP, Barrand MA, Callingham BA. Caffeine: its effect on catecholamines and metabolism in lean and obese humans. Clin Sci (Lond) 1981;60:527-35.   DOI
34 Chumark P, Khunawat P, Sanvarinda Y, Phornchirasilp S, Morales NP, Phivthong-Ngam L, Ratanachamnong P, Srisawat S, Pongrapeeporn KU. The in vitro and ex vivo antioxidant properties, hypolipidaemic and antiatherosclerotic activities of water extract of Moringa oleifera Lam. leaves. J Ethnopharmacol 2008;116:439-46.   DOI   ScienceOn
35 Wei AH, Zhou DN, Ruan JL, Cai YL, Xiong CM, Li MX. Characterisation of phenols and antioxidant and hypolipidaemic activities of Lethariella cladonioides. J Sci Food Agric 2012;92: 373-9.   DOI   ScienceOn
36 Chen N, Bezzina R, Hinch E, Lewandowski PA, Cameron-Smith D, Mathai ML, Jois M, Sinclair AJ, Begg DP, Wark JD, Weisinger HS, Weisinger RS. Green tea, black tea, and epigallocatechin modify body composition, improve glucose tolerance, and differentially alter metabolic gene expression in rats fed a high-fat diet. Nutr Res 2009;29:784-93.   DOI   ScienceOn
37 Shi Y, Burn P. Lipid metabolic enzymes: emerging drug targets for the treatment of obesity. Nat Rev Drug Discov 2004;3:695- 710.   DOI   ScienceOn