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http://dx.doi.org/10.4162/nrp.2009.3.4.279

Grape skin improves antioxidant capacity in rats fed a high fat diet  

Lee, Su-Jin (Department of Food and Nutrition, Yeungnam University)
Choi, Soo-Kyong (Department of Food and Nutrition, Yeungnam University)
Seo, Jung-Sook (Department of Food and Nutrition, Yeungnam University)
Publication Information
Nutrition Research and Practice / v.3, no.4, 2009 , pp. 279-285 More about this Journal
Abstract
This study was conducted to investigate the effect of dietary grape skin on lipid peroxidation and antioxidant defense system in rats fed high fat diet. The Sprague-Dawley rats were fed either control (5% fat) diet or high fat (25% fat) diet which was based on AIN-93 diet for 2 weeks, and then they were grouped as control group (C), control + 5% grape skin group (CS), high-fat group (HF), high fat + 5% grape skin group (HFS) with 10 rats each and fed corresponding diets for 4 weeks. The hepatic thiobarbituric acid reacting substances (TBARS) were increased in high fat group as compared with control group, but reduced by grape skin. The serum total antioxidant status, and activities of hepatic catalase and superoxide dismutase, xanthine oxidase and glucose-6-phosphatase were increased by supplementation of grape skin. Glutathione peroxidase activity was significantly higher in CS group than in C group. Grape skin feeding tended to increase the concentration of total glutathione, especially in control group. The ratio of reduced glutathione to oxidized glutathione was lower in high fat groups than in control groups. The ratio was increased by dietary supplementation of grape skin in control group. These results suggest that dietary supplementation of grape skin would be effective on protection of oxidative damage by lipid peroxidation through improvement of antioxidant defense system in rats fed high fat diet as well as rats with low fat diet.
Keywords
Grape skin; antioxidant; obesity; high fat; rat;
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1 Acquaviva R, Russo A, Galvano F, Galvano G, Barcellona ML, Bolti GL & Vanella A (2003). Cyanidin and cyanidin 3-O-$\beta$-D-g lucoside as DNA cleavage protectors and antioxidants. Cell Biol Toxicol 19:243-252   DOI   ScienceOn
2 Akhileshwar V, Patel SP & Katyare SS (2007). Diabetic cardiomyopathy and reactive oxygen species related parameters in male and female rats: A comparative study. Indian J Clin Biochem 22:84-90   DOI   ScienceOn
3 Amirkhizi F, Siassi F, Minaie S, Djalali M, Rahimi A & Chamari M (2007). Is obesity associated with increased plasma lipid peroxidation and oxidative stress in women? ARYA Atherosclerosis Journal 2:189-192
4 Campolo J, Maria RD, Caruso R, Accinni R, Turazza F, Parolini M, Roubina E, Chiara BD, Cighetti G, Frigerio M, Vitali E & Parodi O (2006). Blood glutathone as independent marker of lipid peroxidation in heart failure. Int J Cardiol 117:45-50   DOI   ScienceOn
5 Fukao H, Ijiri Y, Miura M, Hashimoto M, Yamashita T, Fukunaga C, Oiwa K, Kawai Y, Suwa M & Yamamoto J (2004). Effect of trans-resveratrol on the thrombogenicity and atherogenicity in apolipoprotein E-deficient and low-density lipoprotein receptordeficient mice. Blood Coagul Fibrinolysis 15:441-446   DOI   ScienceOn
6 Hsu CL & Yen GC (2007). Effect of gallic acid on high fat diet-induced dyslipidaemia, hepatosteatosis and oxidative stress in rats. Br J Nutr 98:727-735   DOI   ScienceOn
7 Miura D, Miura Y & Yagasaki K (2003). Hypolipidemic action of dietary resveratrol, a phytoalexin in grapes and red wine, in hepatoma-bearing rats. Life Sci 73:1393-1400   DOI   ScienceOn
8 Rocha KK, Souza GA, Ebaid GX, Seiva FRF, Cataneo AC & Novelli ELB (2009). Resveratrol toxicity: Effects on risk factors for atherosclerosis and hepatic oxidatice stress in standard an high-fat diets. Food Chem Toxicol 47:1362-1367   DOI   ScienceOn
9 West DB & York B (1998). Dietary fat, genetic predisposition, and obesity: lessons from animal models. Am J Clin Nutr 67:505s-5012s   DOI
10 Makris DP, Boskou G & Andrikopoulos NK (2007). Polyphenolic content and in vitro antioxidant characteristics of wine industry and other agri-food solid waste extracts. J Food Compost Anal 20:125-132   DOI   ScienceOn
11 Young JF, Dragsted LO, Daneshvar B, Lauridsen ST, Hansen M & Sandstrom B (2000). The effect of grape skin extract on oxidative status. Br J Nutr 84:505-513   DOI
12 Frederiksen H, Mortensen A, Schroder M, Frandsen H, Bysted A, Kunthsen P & Rasmussen SE (2007). Effects of red grape skin and seed extract supplementation on atherosclerosis in watanabe heritable hyperlipidemic rabbits. Mol Nutr Food Res 51:564-571   DOI   ScienceOn
13 Negro C, Tommasi L & Miceli A (2003). Phenolic compounds and antioxidant activity from red grape marc extracts. Bioresour Technol 87:41-44   DOI   ScienceOn
14 Falchi M, Bertelli A, Scalzo R, Morassut M, Morelli R, Das S, Cui J & Das DK (2006). Comparison of cardioprotective abilities between the flesh and skin of grapes. J Agric Food Chem 54:6613-6622   DOI   ScienceOn
15 Madeira SV, Auger C, Anselm E, Chataigneau M, Chataigneau T, Moura RS & Schini-Kerth VB (2009). eNOS activation induced by a polyphenol-rich grape skin extract in porcine coronary arteries. J Vasc Res 46:406-416   DOI   ScienceOn
16 Re R, Pellegrini N, Proteggente A, Pannala A, Yang M & Rice-Evans C (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26:1231-1237   DOI   ScienceOn
17 Hogeboom GH (1955). Fractionation of cell components of animal tissues. Meth Enzymol 1:16-19   DOI
18 Olusi SO (2002). Obesity is an independent risk factor for plasma lipid peroxidation and depletion of erythrocyte cytoprotectic enzymes in humans. Int J Obes 26:1159-1164   DOI   ScienceOn
19 Aebi H (1974). Catalase. In: Bergmeyer HU (Eds.), Methods of Enzymatic Analysis, 11:673-684. Academic Press, New York. USA
20 Yassa N, Razavi BH & Hadjiakhoondi A (2008). Free radical scavenging and lipid peroxidation activity of the shahani black grape. Pak J Biol Sci 11:2513-2516   DOI
21 Miller NJ, Rice0Ebans C, Davies MJ, Gopinathan V & Milner A (1993). A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin Sci 84:407-412   DOI
22 FAO (2005). STAT database. www.Fao.org. Accessed on 7/20/2007
23 Oben JE, Enyegue DM, Fomekong GI, Soukontoua YB & Agbor GA (2007). The effect of Cissus quadrangularis (GQR-300) and a Cissus formulation (CORE) on obesity and obesity-induced oxidative stress. Lipids Health Dis 6:4-12   DOI   ScienceOn
24 Strip F & della Corte E (1969). The regulation of rat liver xanthine oxidase; Conversion in vitro of the enzyme activity from dehydrogenase (Type D) to oxidase (Type O). J Biol Chem 244:3855-3863
25 Belleville J (2002). The Franch paradox: Possible involvemenet of ethanol in the protective effect against cardiovascular disease. Nutrition 18:173-177   DOI   ScienceOn
26 Scarlatti F, Sala G, Somenzi G, Signorelli P, Sacchi N & Ghidoni R (2003). Resveratrol induces growth inhibition and apoptosis in metastatic breast cancer cells via de novo ceramide signaling. FASEB J 17:2339-2341   DOI   ScienceOn
27 Su H, Hung L & Chen J (2006). Resveratrol, a red wine antioxidant, possesses an insulin-like effect in streptozotocin-induced diabetic rats. Am J Physiol Endocrinol Metab 290:E1339-E1346   DOI   ScienceOn
28 Zhang X, Dong F, Ren J, Driscoll MJ & Culver B (2005). High dietary fat induces NADPH oxidase-associated oxidative stress and inflammation in rat cerebral cortex. Exp Neurol 191:318-325   DOI   ScienceOn
29 Beltowski J, Wojcicka G, Gorny D & Marciniak A (2000). The effect of dietary-induced obesity on lipid peroxidation, antioxidant enzymes and total plasma antioxidant capacity. J Physiol Pharmacol 51:883-896
30 Fang YZ, Yang S & Wu G (2002). Free radicals, antioxidants and nutrition. Nutrition 18:872-879   DOI   ScienceOn
31 Kasdallah-Grissa A, Mornagui B, Aouani E, Hammami M, Gharbi N, Kamoun A & El-Fazaa S (2006). Protective effect of resveratrol on ethanol-induced lipid peroxidation in rats. Alcohol Alcohol 41:236-239   DOI   ScienceOn
32 Lowry OH, Rosebrough NJ, Farr AL & Randall RJ (1951). Protein measurement with the Folin phenol reagent. J Biol Chem 193:265-275
33 Feillet-Coudray C, Sutra T, Fouret G, Ramos J, Wrutniak-Cabello C, Cabello G, Cristol JP & Coudray C (2009). Oxidative stress in rats fed a high-fat high-sucrose diet and preventive effect of polyphenols: Involvement of mitochondrial and NAD(P)H oxidase systems. Free Radic Biol Med 46:624-232   DOI   ScienceOn
34 Bae HS (2008). Effect of grape skin with resveratrol emplification on lipid metabolism and antioxidative system in rats fed high cholesterol diet. MS thesis, Yeungnam University, Gyeongbuk. Republic of Korea
35 Baginski ES, Foa PP & Zak B (1983). Glucose-6-phosphatase. In: Bergmeyer HU (Eds.), Methods of Enzymatic Analysis, 2:876-880. Academic Press, New York. USA
36 Furukawa S, Fujita T, Shimabukuro M, Lwaki M, Yamada Y, Nakajima Y, Nakayama O, Makishima M, Matsuda M & Shimomura I (2004). Increased oxidative stress in obesity and its impact on metabolic syndrome. J Clin Invest 114:1752-1761   DOI   ScienceOn
37 Sener G, Topaloglu N, Sehirli O, Ercan F & Gedik N (2007). Resveratrol alleviates bleomycin-induced lung injury in rats. Pulm Pharmacol Ther 20:642-649   DOI   ScienceOn
38 Vasdev S & Gill V (2005). Antioxidants in the treatment of hypertension. Int J Angiol 14:60-73   DOI
39 Yagi K (1976). A simple fluorometric assay for lipoperoxide in blood plasma. Biochem Med 15:212-216   DOI   ScienceOn
40 Aggarwal BB, Bhardwaj A, Aggarwal RS, Seeram NP, Shishodia S & Takada Y (2004). Role of resveratrol in prevention and therapy of cancer: Preclinical and clinical studies. Anticancer Res 24:3-11
41 Habig WH, Pabst MJ & Jakoby WB (1974). Glutathione-Stransferase. The first enzymatic step in mercapturic acid formation. J Biol Chem 249(22):7130-7139
42 Zhang Y, Liu Y, Wang T, Li B, Li H, Wang Z & Yang B (2006). Resveratrol, a natural ingredient of grape skin: Antiarrhythmic efficacy and ionic mechanisms. Biochem Biophys Res Commun 340:1192-1199   DOI   ScienceOn
43 Cho YJ, Kim JE, Chun HS, Kim CT, Kim SS & Kim CJ (2003). Contents of resveratrol in different parts of grapes. Korean Journal of Food Science and Technoloy 35:306-308   과학기술학회마을   ScienceOn
44 Evans P & Halliwell B (2001). Micronutrients: Oxidabt/antioxidant status. Br J Nutr 85:S67-S74   DOI
45 Singleton VL, Joseph A & Possi JR (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 16:144-158
46 Bray GA, Paeratakul S & Popkin BM (2004). Dietary fat and obetisy: a review of animal, clinical and epidemiological studies. Physiology & Behavior 83:549-555   DOI   ScienceOn
47 Pelicano H, Carney D & Huang P (2004). ROS stress in cancer cells and therapeutic implications. Drug Resist Updat 7:97-110   DOI   ScienceOn
48 Schneider Y, Vincent F, Duranton B, Badolo L, Gosse F, Bergmann C, Seiler N & Raul F (2000). Anti-proliferative effect of resveratrol, a natural component of grapes and wine, on human colonic cancer cells. Cancer Lett 158:85-91   DOI   ScienceOn
49 Bralley EE, Hargrove JL, Greenspan P & Hartle DK (2007). Topical anti-inflammatory activities of Vitis rotundifolia(Muscadine grape) extracts in the tetradecanoylphorbol acetate model of ear inflammation. J Med Food 10:636-642   DOI
50 Decorde K, Teissedre PL, Sutra T, Ventura E, Cristol JP & Rouanet JM (2009). Chardonnay grape seed procyanidin extract supplementation prevents high-fat diet-induced obesity in hamsters by improving adipokine imbalance and oxidative stress markers. Mol Nutr Food Res 53:659-666   DOI   ScienceOn
51 Tsuda T, Horio F, Uchida K, Aoki H & Osawa T (2003). Dietary cyanidin 3-O-$\beta$-D-glucoside-rich purple corn color prevents obesity and ameliorates hyperglycemia in mice. J Nutr 133:2125-2130   DOI
52 Ramirez-Tortosa C, Andersen OM, Gardner PT, Morrice PC, Wood SG, Duthie SJ, Collins AR & Duthie GG (2001). Anthocyanin-rich extract decreases indices of lipid peroxidation and DNA damage in vitamin E-depleted rats. Free Radic Biol Med 31:1033-1037   DOI   ScienceOn
53 Sies H (1999). Glutathione and its role in cellular functions. Free Radic Biol Med 27:916-921   DOI   ScienceOn
54 Yunoki K, Sasaki G, Tokuji Y, Kinoshita M, Naito A, Aida K & Ohnishi M (2008). Effect of dietary wine pomace eztract and oleanolic acid on plasma lipids in rats fed high-fat diet and its DNA microarray analysis. J Agric Food Chem 56:12052-12058   DOI   ScienceOn
55 Caballero B (2007). The global epidemic of obesity: An overview. Epidemiol Rev 29:1-5   DOI   ScienceOn
56 Delmas D, Jannin B & Latruffe N (2005). Resveratrol: Preventing properties against vascular alterations and ageing. Mol Nutr Food Res 49:377-395   DOI   ScienceOn
57 Ohkawa H, Ohishi N & Yagi K (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351-358   DOI   ScienceOn
58 Cui J, Juhasz B, Tosaki A, Maulik N & Das DK (2002). Cardioprotection with grape. J Cardiovasc Pharmacol 40:762-769   DOI   ScienceOn
59 Paglia DE & Valentine WN (1967). Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70:158-169
60 Anderson ME (1985). Determination of glutathione and glutathione disulfide in biological samples. Meth Enzymol 113:548-555   DOI
61 Marklund S & Marklund G (1974). Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47:469-474   DOI   ScienceOn