Browse > Article

Effects of a Selective COX-2 Inhibitor Celecoxib and Soy-Isoflavones on Molecular Markers Related to Apoptosis, and COX-2 and Mapkinase Expression in Estrogen-Fed Rats  

Kim, Tae-Kyung (Department of Food and Nutrition, Hannam University)
Park, Ock Jin (Department of Food and Nutrition, Hannam University)
Publication Information
Nutritional Sciences / v.8, no.1, 2005 , pp. 16-22 More about this Journal
Abstract
The present study examined the effects of cyclooxygenase-2 (COX-2) inhibitor celecoxib or soy-isoflavones in the presence of estrogen on apoptosis related gene expression, COX-2 and mapkinase in 48-week old female rats. Expressions of bel-2 and bax proteins, which are known to be involved in the regulation of apoptosis, were investigated in mammary glands and heart tissues. The elevated expression of bel-2 expression was observed in mammary glands of celecoxib supplemented rats as well as soy-isoflavones. The mammary glands bel-2/bax ratio was found to be higher in celecoxib or soy-isoflavones supplemented rats. However, in heart tissues, expression of bel-2 and bax was in the order of control, celecoxib and soy-isoflavones. The up-regulation of COX-2 was observed in celecoxib or soy-isoflavones in mammary glands. 'The similar trend was not displayed with the mapkinase expression. In heart tissues, the down-regulation of COX-2 as well as mapkinase was observed in celecoxib or soy-isoflavones supplemented rats. Soy-isoflavones and celecoxib both had a similar regulatory pattern of bel-2, bax and COX-2 in mammary glands, and in heart tissues, only COX-2 exhibited a similar down-regulatory properly. These findings revealed that in estrogen sufficient state, celecoxib and soy-isoflavones might not exhibit proapoptotic potential or COX-2 inhibition in normal mammary glands.
Keywords
Soy-isoflavones; Celecoxib; Molecular markers related to apoptosis; Mature estrogen-supplemented rat;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Ziegler RG, Hoover RN, Pike MC. Migration patterns and breast cancer risk in Asian-American women. J Nat Cancer Inst 85:1819-1827, 1993   DOI   ScienceOn
2 Trifan OC, Hla T. Cyclooxygenase-2 modulates cellular growth and promotes tumorigenesis. J Cell Mol Med 7:207-222, 2003   DOI   ScienceOn
3 Kismet K, Akay MT, Abbasoglu O, Ercan A. Celecoxib: a potent cyclooxygenase-2 inhibitor in cancer prevention. Cancer Detect Prev 28:127-142, 2004   DOI   ScienceOn
4 Ding H, Han C, Zhu J, Chen CS, D'Ambrosio SM. Celecoxib derivatives induce apoptosis via the disruption of mitochondrial membrane potential and activation of caspase 9. Int J Cancer 113:803-810, 2005   DOI   ScienceOn
5 The Endogenous Hormones and Breast Cancer Collaborative Group. Endogenous sex hormones and breast cancer risk in post-menopausal women: reanalysis of nine prospective studies. J Natl Cancer lnst 94:606-616,2002   DOI   ScienceOn
6 Martin PM, Horwitz KB, Ryan DS, McGuire WL. Phytoestrogen interaction with estrogen receptors in human breast cancer cells. Endocrinol 103:1860-1867, 1978   DOI   ScienceOn
7 Kuiper GGJM, Lemmen JG, Carlsson B, Corton JC, Safe SH, Van der Saag PT, Van der Burg B, Gustafsson J. Interaction of estrogenic chemicals and phytoestrogens with estrogen receptor ${\beta}$. Endocrinol 139:4252-4263, 1998   DOI   ScienceOn
8 Wong E, Huang J, Tagari P, Riendear D. Effects of COX-2 inhibitors on aortic prostacyclin production in cholesterol-fed rabbits. Atherosclerosis 157:393-402, 2001   DOI   ScienceOn
9 Ghanam K, Lavagna C, Burgaud J-L, Javellaud J, Ea-Kim L, Oudart N. Involvement of cyclooxygenase 2 in the protective effect of 17 ${\beta}$-estradiol in hypercholesterolemic rabbit aorta. Biochem Biophy Resear Comm 275:696-703, 2000   DOI   PUBMED   ScienceOn
10 Adlercreutz H, Mazur W. Phyto-estrogens and western diseases. Ann Med 29:95-120, 1997
11 Ge T, Hughes H, Junquero DC, Wu KK, Vanhoutte PM, Boulanger CM. Endothelium-dependent contractions are associated with both augmented expression of prostaglandin H synthase-1 and hypersensitivity to prostaglandin $H_{2}$ in the SHR aorta. Cir Res 76:1003-1010, 1993
12 Wang TTY, Sathyamoorthy N, Phang JM. Molecular effects of genistein on estrogen receptor mediated pathways. Carcinogenesis 17:271-275, 1996   DOI   ScienceOn
13 Baker CS, Hall RJ, Evans TJ. Cyclooxygenase-2 is widely expressed in atherosclerotic lesions affecting native and transplanted human coronary arteries and colocalizes with inducible nitric oxide synthase and nitrotyrosine particularly in macrophages. Arterioscler Thromb Vasc BioI 19:646-655, 1999   DOI   ScienceOn
14 Constatinou AI, Kamath N, Murley JS. Genistein inactivates bcl-2, delays the G2/M phase of the cell cycle, and induces apoptosis of human breast adenocarcinoma MCF-7 cells. Eur J Cancer 34:1927-1934, 1998   DOI   ScienceOn
15 DuBois RN, Abramson SB, Crofford L, Gupta RA, Simon LS, Van De Putte LB, Lipsky PE. Cyclooxygenase in biology and diesease. FASEB J 12:1063-1073, 1998
16 Williams CS, Mann M, DuBois RN. The role of cyclo­oxygenases in inflammation, cancer and development. Oncogene 18:7908-7916, 1999   DOI   ScienceOn
17 Breinholt V, Hossaini A, Svendsen G, Brouwer C, Nielsen SE. Estrogenic activity of flavonoids in mice. The importance of estrogen receptor distribution, metabolism and bioavailability. Food Chem Toxicol 38:555-564, 2000   DOI   ScienceOn
18 Tshilias J, Kapusta L, Slingerland J. The prognostic signi­ficance of altered cyclin-dependent kinase inhibitors in human cancer. Ann Rev Med 50:401-423, 1999   DOI   ScienceOn
19 Adlercreutz H, Honjo H, Higashi A, Fotsis T, Hamalainen E, Hasegawa T, Okada H. Urinary excretion of lignins and isoflavonoid phytoestrogens in Japanese men and women consuming a traditional diet. Am J Clin Nutr 54:1093-1100,1991
20 Zheng W, Dai Q, Custer L, Shu XO, Wen WQ, Jin F, Franke AA. Urinary excretion of isoflavonoids and the risk of breast cancer. Cancer Epidemiol Biomark Prev 8:35-40, 1999
21 Hla T, Ristimaki A, Appleby S, Barriocanal JG. Cyclooxyg­enase gene expression in inflammation and angiogenesis. Ann N Y Acad Sci 696: 197-204, 1993
22 Kroemer G. The proto-oncogene BcI-2 and its role in regulation apoptosis. Nature Med 3:614-620, 1997   DOI   PUBMED   ScienceOn
23 Cao Y, Prescott SM. Many actions of Cyclooxygenase-2 in cellular dynamics and in cancer. J Cell Physiol 190:279-286, 2002   DOI   ScienceOn
24 Xu ZW, Friess H, buchler MW, Solioz M. Overexpression of Bax sensitizes human pancreatic cancer cells to apoptosis induced by chemotherapeutic agents. Cancer Chemother Pharmacol 49:504-510, 2002   DOI   ScienceOn
25 Kohli E, Zalrid M, Rogan E, Cavalieri EL. Greater reactivity of estradiol-3,4-quinone versus estradiol-2,3-quinone with DNA in the formation of depurinating adducts. Proc Am Assoc Cancer Res 44:180, 2003
26 Thom DM, Gardner CD, Haskell WL. Potential health benefits of dietary phytoestrogens: A review of the clinical, epidemiological, and mechanistic evidence. J Clin Endocrinol Metab 83:2223-2235, 1998   DOI   ScienceOn
27 Shao A, Alpaugh ML, Fontana JA, Barsky SH. Geinstein inhibits proliferation similarly in estrogen receptor-positive and negative human breast carcinoma cell lines characterized by p21WAF1/CIP1 induction, G2/M arrest, and apoptosis. J Cell Biochem 69:44-54, 1998   DOI   ScienceOn
28 Leung LK, Wang TT. Bcl-2 is not reduced in the death of MCF-7 cells at low genistein concentration. J Nutr 130:2922-2926, 2000
29 Luscher TF, Aarhus LL, Vanhoutte PM. Indomethacin improves the impaired endothelium-dependent relaxations in small mesenteric arteries of the spontaneously hypertensive rats. Am J Hypertens 3:55-58, 1990
30 Brzozowski AM, Pike CW, Pauter Z, Hubbard RE, Bonn T, Engstrom O, Ohman L, Greene GL, Gustafsson J, Carlquist M. Molecular basis of agonism and antagonism in the oestrogen receptor. Nature 389:753-758, 1997   DOI   ScienceOn
31 Routledge EJ, White R, Parker MG, Sumpter JP. Differential effects of xenoestrogens on coactivator recruitment by estrogen receptor (ER) ${\alpha}$ and $ER{\beta}$. J BioI Chem 275: 35968-35993, 2000
32 Li JJ, Li SA. Estrogen carcinogenesis in hamster tissues: role of metabolism. Fed Proc 46:1858-1863, 1987
33 Reed JC BcI-2:Prevention of apoptosis as a mechanism of drug resistance. Hematol Oncol Clin North Am 9:451-474, 1995
34 Toniolo PG, Levitz M, Zeleniuch-Jacquotte A, Banerjee S, Koenig KL, Shore RE, Strax P, Pasternack BS. A prospective study of endogeous estrogens and breast cancer in postmenopausal women. J Natl Cancer Inst 67:190-197, 1995
35 Liehr JG, Fang WF, Sirbasku DA, Ari-Ulubelen A. Carcinogenicity of catechol estrogens in Syrian hamsters. J Steriod Biochem 24:353-356, 1986   DOI   ScienceOn
36 Harvell DM, Strecker TE, Tochacek M, Xie B, Pennington KL, McComb RD, Roy SK, Shull JD. Rat strain-specific actions of $17{\beta}$-estradiol in the mammary gland: correlation between estrogen-induced lobuloalveolar hyperplasia and susceptibility to estrogen-induced mammary cancers. Proc Natl Acad Sci, 97:2779-2784, 2000
37 Dyer SM, Taylor DA, Bexis S, Hime NJ, Frewin DB, Head RJ. Identification of a non-endothelial cell thromboxane-like constrictor response and its interaction with the renin-angiotensin system in the aorta of spontaneously hypertensive rats. J Vasc Res 31:5-60, 1994
38 Lee H P, Gourley L, Duffy SW, Esteve J, Lee J, Day NE. Dietary effects on breast cancer risk in Singapore. Lancet 336: 1197-1200, 1991
39 Thomas HV, Key TJ, Allen DS, Moore JW, Dowsett M, Fentiman IS, Wang DY. A prospective study of endogenous serum hormone concentrations and breast cancer risk in post-menopausal women on the island of Guernsey. Br J Cancer 76:401-405, 1997   DOI   ScienceOn
40 Allred CD, Allred KF, Ju YH, Virant SM, Helferich WG. Soy diets containing varying amounts of genistein stimulate growth of estrogen-dependent (MCF-7) tumors in a dose­dependent manner. Cancer Res 61:45-50, 2001
41 Davies P, Bailey PJ, Goldenberg MM, Ford-Hutchinson A W. The role of arachidonic acid oxygenation in pain and inflam­mation. Ann Rev lmmunol 2:335-357, 1984   DOI   ScienceOn
42 Li Y, Upadhyay S, Bhuiyan M, Sarkar FH. Induction of apoptosis in breast cancer cells MDA-MB-231 by genistein. Oncogene 18:3166-3172, 1999   DOI   ScienceOn
43 Shutt DA, Cox I. Steroid and phytoestrogen binding to sheep uterine receptors in vitro. J Endocrinol 52:299-310, 1972   DOI   ScienceOn
44 Barkhem T, Carlsson B, Nilsson Y, Enmark E, Fustafsson J, Nilsson S. Differential response of estrogen receptor ${\alpha}$ and estrogen receptor ${\beta}$ to partial estrogen agonists/antagonists. Mole Pharm 54:105-112, 1998
45 Ingram D, Sanders K, Kolybaba M, Lopez D. Case-control study of phyto-oestrogens and breast cancer. Lancet 350:990-994,1997   DOI   ScienceOn
46 Prall OW, Rogan EM, Sutherland RL. Estrogen regulation of cell cycle progression in breast cancer cells. J Steroid Biochem Mol BioI 65:169-174, 1998   DOI   ScienceOn
47 Knight DC, Eden JA. A review of the clinical effects of phytoestrogens. Obstet Gynecol 87:897-904, 1996