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Inhibitory Effects of (-)Epigallocatechin Gallate and Quercetin on Phorbol 12-Myristate 13-Acetate-Induced Secretion of Metalloproteinase-2 and Metalloproteinase-9  

Kang Sang-Wook (Department of Food and Nutrition and Institute of Nutrition Research, Hallym University)
Choi Yean-Jung (Department of Food and Nutrition and Institute of Nutrition Research, Hallym University)
Choi Jung-Suk (Department of Food and Nutrition and Institute of Nutrition Research, Hallym University)
Kwon Hyang-Mi (Department of Food and Nutrition and Institute of Nutrition Research, Hallym University)
Bae Ji-Young (Department of Food and Nutrition and Institute of Nutrition Research, Hallym University)
Park Eun-Hee (Department of Food and Nutrition and Institute of Nutrition Research, Hallym University)
Ji Geun-Eog (Department of Food and Nutrition, Seoul National University)
Kang Il-Jun (Department of Food and Nutrition and Institute of Nutrition Research, Hallym University)
Kang Young-Hee (Department of Food and Nutrition and Institute of Nutrition Research, Hallym University)
Publication Information
Nutritional Sciences / v.9, no.3, 2006 , pp. 145-151 More about this Journal
Abstract
Matrix metalloproteinases (MMP) play an important role in the extracellular matrix (ECM) degradation undetphysiological and pathological conditions. The present study examined the influence of (-)epigallocatechin gallate and quercetin on phorbol-12-myristate 13-acetate (PMA)-induced secretion of MMP-2 and MMP-9, when human umbilical vein endothelial cells (HUVEC) were treated with (-)epigallocatechin gallate and quercetin at supraphysiological concentrations of $25{\mu}mol/L$. No cytotoxicity was observed by MIT assay in response to a treatment with PMA in the presence of (-)epigallocatechin gallate and quercetin. Western blot analysis and gelatin zymography revealed that exposure of HUVEC to PMA enhanced the levels and gelatinolytic activities of pro and active forms of MMP-2 and active form of MMP-9. (-)Epigallocatechin gallate attenuated PMA-stimulated secretion of active forms of MMP-2 and MMP-9 concomitantly with a loss of activities of these enzymes, which was related to the decreased mRNA levels of MMP. Quercetin was more potent than (-)epigallocatechin gallate in alleviating MMP-9 protein secretion and activity with a decrease in MMP-9 mRNA accumulation. Taken together, the results indicated that (-)epigallocatechin gallte and quercetin exhibited inhibitory effects on MMP activity and may qualify as chemopreventive and cardiovascular protective agents.
Keywords
Metalloproteinase-2; Matrix metalloproteinase-9; Phorbol-12-myristate 13-acetate; (-)Epigallocatechin gallate; Quercetin;
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