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http://dx.doi.org/10.5478/MSL.2019.10.4.117

Electron Capture Dissociation Mass Spectrometry for Gaseous Protonated Melittin Ions and Its Single Amino Acid Substituted Variants  

Yu, Seonghyun (Department of Chemistry, Sogang University)
Jang, Hwa-yong (Department of Chemistry, Sogang University)
Oh, Han Bin (Department of Chemistry, Sogang University)
Publication Information
Mass Spectrometry Letters / v.10, no.4, 2019 , pp. 117-122 More about this Journal
Abstract
This study demonstrated the sensitivity of electron capture dissociation mass spectrometry (ECD-MS) to probe subtle conformational changes in gaseous melittin ions induced by the substitution of an amino acid. ECD-MS was performed for triply and quadruply-protonated melittin and its variants obtained by a single amino acid substitution, namely, D-Pro14, Pro14Ala, and Leu13Ala. Although native triply-protonted melittin showed only a few peptide backbone cleavage products, the D-Pro14 and Pro14Ala variants exhibited extensive backbone fragments, suggesting the occurrence of a significant structural or conformational change induced by a single amino acid substitution at Pro14. On the contrary, the substitution at Leu13, namely Leu13Ala (+3), did not cause significant changes in the ECD backbone fragmentation pattern. Thus, the sensitivity of ECD-MS is demonstrated to be good enough to probe the aforementioned conformational change in melittin.
Keywords
Electron Capture Dissociation; Melittin; Amino Acid Substitution; Chiral Inversion; Mass Spectrometry;
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