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http://dx.doi.org/10.5478/MSL.2016.7.4.85

Nitrated Proteome in Human Embryonic Stem Cells  

Kang, Jeong Won (Department of Applied Chemistry, Kyung Hee University)
Hwang, Daehee (Department of New Biology and Center for Plant Aging Research, Institute for Basic Science, DGIST)
Kim, Kwang Pyo (Department of Applied Chemistry, Kyung Hee University)
Publication Information
Mass Spectrometry Letters / v.7, no.4, 2016 , pp. 85-90 More about this Journal
Abstract
Post-translational modifications (PTMs) of proteins regulate self-renewal and differentiation in embryonic stem cells (ESCs). Nitration of tyrosine residues of proteins in ESCs modulates their downstream pathways, which can affect self-renewal and differentiation. However, protein tyrosine nitration (PTN) in ESCs has been rarely studied. We reviewed 23 nitrated sites in stem cell proteins. Functional enrichment analysis showed that these nitrated proteins are involved in signal transduction, cell adhesion and migration, and cell proliferation in ESCs. Comparison between the nitrated and known phosphorylated sites revealed that 7 nitrated sites had overlapping phosphorylated sites, indicating functional links of PTNs to their associated signaling pathways in ESCs. Therefore, nitrated proteome provides a basis for understanding potential roles of PTN in self-renewal and differentiation of ESCs.
Keywords
Tyrosine nitration; Nitrotyrosine enrichment; LC-MS/MS; Proteomics; Embryonic stem cells;
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