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http://dx.doi.org/10.5478/MSL.2014.5.4.115

Solid Phase Extraction of Phospholipids from Brazil Nut (Bertholletia excelsa) and Their Characterization by Mass Spectrometry Analysis

Lima, Bruna R. De (Department of Chemistry, Federal University of Amazonas)
Silva, Felipe M.A. Da (Department of Chemistry, Federal University of Amazonas)
Koolen, Hector H.F. (Department of Chemistry, Federal University of Amazonas)
Almeida, Richardson A. De (Department of Chemistry, Federal University of Amazonas)
Souza, Afonso D.L. De (Department of Chemistry, Federal University of Amazonas)

Publication Information

Mass Spectrometry Letters / v.5, no.4, 2014 , pp. 115-119 More about this Journal

Abstract

The Brazil nut (Bertholletia excelsa - Lecythidaceae) is considered a product with high economic value, being a food widely appreciated for its nutritional qualities. Although previous studies have reported the biochemical composition of Brazil nut oil, the knowledge regarding the phospholipid composition exhibits a disagreement: the composition of fatty acids present in the structures of phospholipids is reported as being different from the composition of the free fatty acids present in the oil. In this work, solid phase extraction (SPE) was employed to provide a fast extraction of the phospholipids from Brazil nuts, in order to compare the phospholipid profile of the in nature nuts and their fatty acids precursor present in the oil. The major phospholipids were characterized by mass spectrometry approach. Their fragmentation pattern through direct infusion electrospray ionization ion-trap tandem mass spectrometry ( $ESI-IT-MS^2$ ) proved to be useful to unequivocal characterization of these substances. High resolution (HR) experiments through ESI using a quadruple time of flight mass spectrometry (QTOF) system were performed to reinforce the identifications.

Keywords

Brazil nuts; Bertholletia excelsa; Phosphatidylinositol; Phosphatidic acid; Mass spectrometry; Solid phase extraction;

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