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http://dx.doi.org/10.5478/MSL.2014.5.1.30

Optimization of Solid Phase Extraction Method for Quantitative Analysis of Perfluorooctanoic Acid in Serum using Liquid Chromatography-Tandem Mass Spectrometry

Lee, Hyun-Seok (Korea Research Institute of Standards and Science (KRISS))
Yim, Yong-Hyeon (Korea Research Institute of Standards and Science (KRISS))

Publication Information

Mass Spectrometry Letters / v.5, no.1, 2014 , pp. 30-33 More about this Journal

Abstract

A solid phase extraction (SPE) method was optimized for the quantitative analysis of perfluorooctanoic acid (PFOA) in serum using hydrophilic-lipophilic balance SPE and LC-MS/MS. Fetal bovine serums spiked with $^{13}C_8$ -PFOA before or after SPE were used as test samples for evaluation of the SPE efficiency. Simultaneous evaluation of matrix effects and absolute SPE recovery for $^{13}C_8$ -PFOA in serum using different sample pre-treatments and SPE conditions allowed optimization of SPE process efficiency with minimal matrix effect and decent SPE recovery. Introduction of protein precipitation as a sample pre-treatment procedure for serum samples before SPE generally decreased matrix effect in LC-MS/MS analysis and provided more stable recovery of PFOA.

Keywords

perfluooctanoic acid; solid phase extraction; fatal bovine serum; LC-MS/MS;

Citations & Related Records

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