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http://dx.doi.org/10.5478/MSL.2012.3.4.93

Quantitative Phosphoproteomics of the Human Neural Stem Cell Differentiation into Oligodendrocyte by Mass Spectrometry  

Cho, Kun (Division of Mass Spectrometry Research, Korea Basic Science Institute)
Kim, Jin Young (Division of Mass Spectrometry Research, Korea Basic Science Institute)
Kim, Eunmin (Division of Mass Spectrometry Research, Korea Basic Science Institute)
Park, Gun Wook (Division of Mass Spectrometry Research, Korea Basic Science Institute)
Kang, Tae Wook (Division of Mass Spectrometry Research, Korea Basic Science Institute)
Yoon, Jung Hae (Research Institute at Nationwide Children's Hospital)
Kim, Seung U. (Department of Medicine, University of British Columbia)
Byun, Kyunghee (Center for Genomics and Proteomics, Lee Gil Ya Cancer and Diabetes Institute, Gachon University of Medicine and Science)
Lee, Bonghee (Center for Genomics and Proteomics, Lee Gil Ya Cancer and Diabetes Institute, Gachon University of Medicine and Science)
Yoo, Jong Shin (Division of Mass Spectrometry Research, Korea Basic Science Institute)
Publication Information
Mass Spectrometry Letters / v.3, no.4, 2012 , pp. 93-100 More about this Journal
Abstract
Cellular processes such as proliferation, differentiation, and adaptation to environmental changes are regulated by protein phosphorylation. In order to enhance the understanding of molecular dynamics for biological process in detail, it is necessary to develop sensitive and comprehensive analytical methods for the determination of protein phosphorylation. Neural stem cells hold great promise for neural repair following an injury or disease. In this study, we made differentiated oligodendrocytes from human neural stem cells using over-expression of olig2 gene. We confirmed using quantitative phosphoproteome analysis approach that combines stable isotope labeling by amino acids in cell culture (SILAC) and $TiO_2$ micro-column for phosphopeptide enrichment with $MS^2$ and $MS^3$ mass spectrometry. We detected 275 phosphopeptides which were modulated at least 2-fold between human neural stem cells and oligodendrocytes. Among them, 23 phosphoproteins were up-regulated in oligodendrocytes and 79 phosphoproteins were up-regulated in F3 cells.
Keywords
Phosphoproteomics; $TiO_2$; SILAC; LTQ-FT MS; Neural Stem Cell (NSC); Oligodendrocyte;
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