Browse > Article
http://dx.doi.org/10.5478/MSL.2011.2.4.084

Microwave-assisted Protein Digestion on Various Locations of a Microplate  

Lee, Ji-Hye (Department of Chemistry, Chungnam National University)
Park, Se-Hwan (Department of Chemistry, Chungnam National University)
Lee, Sun-Young (Department of Chemistry, Chungnam National University)
Hong, Jang-Mi (Department of Chemistry, Chungnam National University)
Park, Kyu-Hwan (Korea Basic Science Institute)
Kim, Hyun-Sik (Korea Basic Science Institute)
Kim, Jeong-Kwon (Department of Chemistry, Chungnam National University)
Publication Information
Mass Spectrometry Letters / v.2, no.4, 2011 , pp. 84-87 More about this Journal
Abstract
The effectiveness of microwave-assisted protein digestion in different well positions of a 96-well microplate was investigated where microwave-assisted protein digestion of bovine serum albumin was performed in 10 different wells of a 96-well microplate in a microwave oven. Similarly increased sequence coverages (~70%) were generally observed for the 10 microwave-assisted protein digestion samples compared to conventional overnight digestion (63%), which is possibly due to higher miscleavage ratios (~53%) of the samples from microwave-assisted protein digestion than conventional overnight digestion (42.1%). The reproducible results of microwave-assisted digestions from different well positions demonstrate the potential of high-throughput analysis of proteins using microwave-assisted protein digestion.
Keywords
Microwave; Mass spectrometry; MALDI; Microplate; Digestion;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Pramanik, B. N.; Mirza, U. A.; Ing, Y. H.; Liu, Y. H.; Bartner, P. L.; Weber, P. C.; Bose, A. K. Protein Sci. 2002, 11, 2676.
2 Lopez-Ferrer, D.; Capelo, J. L.; Vazquez, J. J. Proteome Res. 2005, 4, 1569.   DOI   ScienceOn
3 Lopez-Ferrer, D.; Petritis, K.; Hixson, K. K.; Heibeck, T. H.; Moore, R. J.; Belov, M. E.; Camp, D. G., 2nd; Smith, R. D. J. Proteome Res. 2008, 7, 3276.   DOI   ScienceOn
4 Shin, S.; Yang, H. J.; Kim, J.; Kim, J. Anal. Biochem. 2011, 414, 125.   DOI   ScienceOn
5 Yang, H. J.; Shin, S.; Kim, J.; Hong, J.; Lee, S.; Kim, J. Rapid Commun. Mass Spectrom. 2011, 25, 88.   DOI   ScienceOn
6 Yang, H. J.; Hong, J.; Lee, S.; Shin, S.; Kim, J.; Kim, J. Rapid Commun. Mass Spectrom. 2010, 24, 901.   DOI   ScienceOn
7 Wu, S. L.; Kim, J.; Bandle, R. W.; Liotta, L.; Petricoin, E.; Karger, B. L. Mol. Cell. Proteomics 2006, 5, 1610.   DOI   ScienceOn
8 Lin, S.; Yun, D.; Qi, D.; Deng, C.; Li, Y.; Zhang, X. J. Proteome Res. 2008, 7, 1297.   DOI   ScienceOn
9 Sun, W.; Gao, S.; Wang, L.; Chen, Y.; Wu, S.; Wang, X.; Zheng, D.; Gao, Y. Mol. Cell. Proteomics 2006, 5, 769.   DOI
10 Ha, N. Y.; Kim, S. H.; Lee, T. G.; Han, S. Y. Langmuir 2011, 27, 10098.   DOI   ScienceOn
11 Wu, S. L.; Kim, J.; Hancock, W. S.; Karger, B. J. Proteome Res. 2005, 4, 1155.   DOI   ScienceOn