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http://dx.doi.org/10.5478/MSL.2011.2.3.061

Development of a Matrix-prespotted Plate for Enhancing the Reproducibility of Serum Glycan Analysis by MALDI-TOF-MS  

Ha, Mi-Young (Applied Surface Technology Inc.)
In, Young-Ha (Applied Surface Technology Inc.)
Maeng, Hye-Sun (Applied Surface Technology Inc.)
Zee, Ok-Pyo (College of Pharmacy, Sungkyunkwan University)
Lee, Jong-Sik (Applied Surface Technology Inc.)
Kim, Yang-Sun (Applied Surface Technology Inc.)
Publication Information
Mass Spectrometry Letters / v.2, no.3, 2011 , pp. 61-64 More about this Journal
Abstract
Matrix Assisted Laser Desorption/Ionization-Time-of-Flight mass spectrometry (MALDI-TOF-MS) is the most widely used MS technique for glycan analysis. However, the poor point-to-point and sample-to-sample reproducibility becomes a limit in glycan biomarker research. A prespotted MALDI plate which overcomes the large crystal formation of 2,5-dihydroxybenzoic acid (DHB) has been developed and applied for glycan analysis. A homogeneous matrix coated surface without a crystal structure was formed on a hydrophilic/ hydrophobic patterned surface using a piezoelectric device. The reproducible MALDI-TOF-MS data have been presented using MALDI imaging of beer glycan as well as serum glycan eluted from 10% and 20% ACN elution fractions. The glycan profile from the serum glycan by MALDI-TOF-MS with a DHB prespotted plate was highly conserved for 10 different spectra and the coefficient of variations of significant ion peaks of MALDI data varies from 3.59 to 19.95.
Keywords
MALDI-TOF-MS; Glycan analysis; Prespotted MALDI plate;
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