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http://dx.doi.org/10.5478/MSL.2011.2.3.061

Development of a Matrix-prespotted Plate for Enhancing the Reproducibility of Serum Glycan Analysis by MALDI-TOF-MS  

Ha, Mi-Young (Applied Surface Technology Inc.)
In, Young-Ha (Applied Surface Technology Inc.)
Maeng, Hye-Sun (Applied Surface Technology Inc.)
Zee, Ok-Pyo (College of Pharmacy, Sungkyunkwan University)
Lee, Jong-Sik (Applied Surface Technology Inc.)
Kim, Yang-Sun (Applied Surface Technology Inc.)
Publication Information
Mass Spectrometry Letters / v.2, no.3, 2011 , pp. 61-64 More about this Journal
Abstract
Matrix Assisted Laser Desorption/Ionization-Time-of-Flight mass spectrometry (MALDI-TOF-MS) is the most widely used MS technique for glycan analysis. However, the poor point-to-point and sample-to-sample reproducibility becomes a limit in glycan biomarker research. A prespotted MALDI plate which overcomes the large crystal formation of 2,5-dihydroxybenzoic acid (DHB) has been developed and applied for glycan analysis. A homogeneous matrix coated surface without a crystal structure was formed on a hydrophilic/ hydrophobic patterned surface using a piezoelectric device. The reproducible MALDI-TOF-MS data have been presented using MALDI imaging of beer glycan as well as serum glycan eluted from 10% and 20% ACN elution fractions. The glycan profile from the serum glycan by MALDI-TOF-MS with a DHB prespotted plate was highly conserved for 10 different spectra and the coefficient of variations of significant ion peaks of MALDI data varies from 3.59 to 19.95.
Keywords
MALDI-TOF-MS; Glycan analysis; Prespotted MALDI plate;
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1 Gusev, A. I.; Wilkinson, W. R.; Proctor, A.; Hercules, D. M. Anal. Chem. 1995, 67, 1034.   DOI   ScienceOn
2 Kirmiiz, C.; Li, B.; An, H. J.; Clowers, B. H.; Chew, H. K.; Lam, K. S.; Ferrige, A.; Alecio, R.; Borowsky, A. D.; Sulaimon, S.; Lebrilla, C. B.; Miyamoto, S. Molecular & Cellular Proteomics 2007, 6, 43.   DOI   ScienceOn
3 Dekker, L. J.; Burgers, P. C.; Guzel, C.; Luider, T. M. Journal of Chromatogaphy B 2007, 847, 62.   DOI   ScienceOn
4 Lee, J. H.; Choi, H.S.; Nasr, K. A.; Ha, M.; Kim, Y.; Frangeoni, J. V. Anal. Chem. 2011, 83 (13), 5283.   DOI   ScienceOn
5 Wikipedia "Glycosylation", http://en.wikipedia.org/wiki/glycoprotein.
6 Turnbull, J. E.; Field, R. A. Nat. Chem. Biol. 2007, 3, 74.   DOI   ScienceOn
7 An, H. J.; Lebrilla, C. B. Mass Spectrom. Rev. 2011, 30, 560.   DOI   ScienceOn
8 Barkauskas, D. A. I; An, H. J.; Kronewitter, S. R.; de Leoz, M. L.; Chew, H. K.; White, R. W. D.; Leiserowitz, G. S.; Miyamoto, S.; Lebrilla, C. B.; Rocke, D. M. Bioinformatics 2009, 25, 251.   DOI   ScienceOn
9 Lebrilla, C. B.; An, H. J. Mol. Biosyst. 2009, 5, 17.   DOI   ScienceOn
10 Kaneshiro, K.; Hukuyama, Y.; Iwamoto, S.; Sekiya, S.; Tanaka, K. Anal. chem. 2011, 83, 3663.   DOI   ScienceOn
11 Harvey, D. J. Mass Spectrom. Rev. 2011, 30, 1.   DOI   ScienceOn
12 Wang, H.; Wang, C. H.; Chin, A.; Taguchi, A.; Taylor, A.; Hanashi, S. Nature protocols, 2011, 6, 253.   DOI   ScienceOn
13 Suzuki, H.; Yamagaki, T.; Tachibana, K. J. Mass Spec. Soc. Japan, 2005, 53, 227.   DOI   ScienceOn
14 Wilkinson, W. R.; Gusev, A. I.; Proctor, A.; Houalla, M.; Hercules, D. M. Fres. J. Anal. Chem. 1977, 357, 241.
15 Kronewitter, S. R.; Leoz, M. L. A.; Peacock, K. S.; McBride, K. R.; An, H. J.; Miyamoto, S.; Leiserowitz, G. S.; Lebrilla, C. B. J. Proteome Res. 2010, 9, 4952.   DOI   ScienceOn
16 Tholey, A.; Heinzle, E. Anal. Bioanal. Chem. 2006, 384, 24.   DOI
17 Nicola, A. J.; Gusev, A. I.; Proctor, A.; Jackson, E. K.; Hercules, D. M. Rapid Commun. Mass Spectrom. 1995, 9, 1164.   DOI   ScienceOn
18 Ha, M.; Maeng, H.; Oh, J.; Kim, Y. 18th International Mass Spectroscopy Conference, Bremen(German), September, 2009.
19 Dekker, L. J.; Dalebout, J. C.; Siccama, I.; Jenster, G.; Sillevis, P. A.; Smitt, T. M. Rapid Commun. Mass Spectrom. 2005, 19, 865.   DOI   ScienceOn