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http://dx.doi.org/10.5478/MSL.2011.2.1.020

Identfication of Phase I and Phase II Metabolites of Hesperetin in Rat Liver Microsomes by Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry  

Kim, Un-Yong (Department of Pharmaceutical analysis, College of Pharmacy, Chung-Ang university)
Han, Sang-Beom (Department of Pharmaceutical analysis, College of Pharmacy, Chung-Ang university)
Kwon, Oh-Seung (Doping Control Center, Korea institute of Science and Technology)
Yoo, Hye-Hyun (Department of Pharmacy, College of Pharmacy, Hanyang University)
Publication Information
Mass Spectrometry Letters / v.2, no.1, 2011 , pp. 20-23 More about this Journal
Abstract
The purpose of this study is to investigate the in vitro metabolism of hesperetin, a bioflavonoid. Hesperetin was incubated with rat liver microsomes in the presence of NADPH and UDP-glucuronic acid for 30 min. The reaction mixture was analyzed by liquid chromatography-ion trap mass spectrometer and the chemical structures of hesperetin metabolites were characterzed based on their MS/MS spectra. As a result, a total of five metabolites were detected in rat liver microsomes. The metabolites were identified as a de-methylated metabolite (eriodictyol), two hesperetin glucuronides, and two eriodictyol glucuronides.
Keywords
Hesperetin; in vitro Metabolism; Rat Liver Microsomes; LC/MS;
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