Browse > Article
http://dx.doi.org/10.5483/BMBRep.2022.55.8.009

2-Undecanone derived from Pseudomonas aeruginosa modulates the neutrophil activity  

Jeong, Yu Sun (Department of Biological Sciences, Sungkyunkwan University)
Huh, Sunghyun (Department of New Biology, DGIST)
Kim, Ji Cheol (Department of Biological Sciences, Sungkyunkwan University)
Park, Ji Ye (Department of Biological Sciences, Sungkyunkwan University)
Lee, ChaeEun (Department of New Biology, DGIST)
Kim, Min-Sik (Department of New Biology, DGIST)
Koo, JaeHyung (Department of New Biology, DGIST)
Bae, Yoe-Sik (Department of Biological Sciences, Sungkyunkwan University)
Publication Information
BMB Reports / v.55, no.8, 2022 , pp. 395-400 More about this Journal
Abstract
Pseudomonas aeruginosa (P. aeruginosa) is a well-known Gramnegative opportunistic pathogen. Neutrophils play key roles in mediating host defense against P. aeruginosa infection. In this study, we identified a metabolite derived from P. aeruginosa that regulates neutrophil activities. Using gas chromatography-mass spectrometry, a markedly increased level of 2-undecanone was identified in the peritoneal fluid of P. aeruginosa-infected mice. 2-Undecanone elicited the activation of neutrophils in a Gαi-phospholipase C pathway. However, 2-undecanone strongly inhibited responses to lipopolysaccharide and bactericidal activity of neutrophils against P. aeruginosa by inducing apoptosis. Our results demonstrate that 2-undecanone from P. aeruginosa limits the innate defense activity of neutrophils, suggesting that the production of inhibitory metabolites is a strategy of P. aeruginosa for escaping the host immune system.
Keywords
2-undecanone; Bacterial metabolite; Neutrophil; Pseudomonas aeruginosa; Volatile organic compound;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Rada B (2017) Interactions between neutrophils and pseudomonas aeruginosa in cystic fibrosis. Pathogens 6, 10
2 Colosimo DA, Kohn JA, Luo PM et al (2019) Mapping interactions of microbial metabolites with human G-protein-coupled receptors. Cell Host Microbe 26, 273-282 e277
3 Kruger P, Saffarzadeh M, Weber AN et al (2015) Neutrophils: between host defence, immune modulation, and tissue injury. PLoS Pathog 11, e1004651
4 Prince LR, Bianchi SM, Vaughan KM et al (2008) Subversion of a lysosomal pathway regulating neutrophil apoptosis by a major bacterial toxin, pyocyanin. J Immunol 180, 3502-3511   DOI
5 Timm CM, Lloyd EP, Egan A, Mariner R and Karig D (2018) Direct growth of bacteria in headspace vials allows for screening of volatiles by gas chromatography mass spectrometry. Front Microbiol 9, 491
6 David N Douda LY, Meraj A Khan, Hartmut Grasemann and Nades Palaniyar (2014) Akt is essential to induce NADPH-dependent NETosis and to switch the neutrophil death to apoptosis. Blood 123, 597-600   DOI
7 Plyuta V, Lipasova V, Popova A et al (2016) Influence of volatile organic compounds emitted by Pseudomonas and Serratia strains on Agrobacterium tumefaciens biofilms. APMIS 124, 586-594   DOI
8 Jung YS, Lee SK, Ok CY et al (2013) Role of CXCR2 on the immune modulating activity of alpha-iso-cubebenol a natural compound isolated from the Schisandra chinensis fruit. Biochem Biophys Res Commun 431, 433-436   DOI
9 Wu X, Li J, Wang S et al (2021) 2-Undecanone protects against fine particle-induced kidney inflammation via inducing mitophagy. J Agric Food Chem 69, 5206-5215   DOI
10 Chen J, Wang W, Shi C and Fang J (2014) A comparative study of sodium houttuyfonate and 2-undecanone for their in vitro and in vivo anti-inflammatory activities and stabilities. Int J Mol Sci 15, 22978-22994   DOI
11 Lee HY, Oh E, Kim SD, Seo JK and Bae YS (2014) Oxidized low-density lipoprotein-induced foam cell formation is mediated by formyl peptide receptor 2. Biochem Biophys Res Commun 443, 1003-1007   DOI
12 Zhai Y, Shao Z, Cai M et al (2018) Multiple modes of nematode control by volatiles of Pseudomonas putida 1A00316 from antarctic soil against Meloidogyne incognita. Front Microbiol 9, 253
13 Lee N, Jae Y, Kim M et al (2020) A pathogen-derived metabolite induces microglial activation via odorant receptors. FEBS J 287, 3841-3870   DOI
14 Bos LD, Sterk PJ and Schultz MJ (2013) Volatile metabolites of pathogens: a systematic review. PLoS Pathog 9, e1003311
15 Stover CK, Pham XQ, Erwin AL et al (2000) Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen. Nature 406, 959-964   DOI
16 Prakash D, Ms A, Radhika B, Venkatesan R, Chalasani SH and Singh V (2021) 1-Undecene from Pseudomonas aeruginosa is an olfactory signal for flight-or-fight response in Caenorhabditis elegans. EMBO J 40, e106938
17 Labows JN, McGinley KJ, Webster GF and Leyden JJ (1980) Headspace analysis of volatile metabolites of Pseudomonas aeruginosa and related species by gas chromatographymass spectrometry. J Clin Microbiol 12, 521-526   DOI
18 Khatua B, Bhattacharya K and Mandal C (2012) Sialoglycoproteins adsorbed by Pseudomonas aeruginosa facilitate their survival by impeding neutrophil extracellular trap through siglec-9. J Leukoc Biol 91, 641-655   DOI
19 Storisteanu DM, Pocock JM, Cowburn AS et al (2017) Evasion of neutrophil extracellular traps by respiratory pathogens. Am J Respir Cell Mol Biol 56, 423-431   DOI
20 F Hartveit ST (1966) Peritoneal fluid volume and the oestrus cycle in mice. Nature 210, 1123-1125   DOI
21 Haifeng Eishingdrelo SK (2013) Minireview: targeting GPCR activated ERK pathways for drug discovery. Curr Chem Cenome Transl Med 7, 9-15
22 Sun L and Ye RD (2012) Role of G protein-coupled receptors in inflammation. Acta Pharmacol Sin 33, 342-350   DOI
23 Papayannopoulos V (2018) Neutrophil extracellular traps in immunity and disease. Nat Rev Immunol 18, 134-147   DOI
24 Pieterse E, Rother N, Yanginlar C, Hilbrands LB and van der Vlag J (2016) Neutrophils discriminate between lipopolysaccharides of different bacterial sources and selectively release neutrophil extracellular traps. Front Immunol 7, 484
25 Widera D, Martinez Aguilar R and Cottrell GS (2019) Toll-like receptor 4 and protease-activated receptor 2 in physiology and pathophysiology of the nervous system: more than just receptor cooperation? Neural Regen Res 14, 1196-1201   DOI
26 Ciesielska A, Matyjek M and Kwiatkowska K (2021) TLR4 and CD14 trafficking and its influence on LPS-induced pro-inflammatory signaling. Cell Mol Life Sci 78, 1233-1261   DOI
27 Liu P, Yang C, Lin S et al (2019) Sodium houttuyfonate inhibits LPSinduced mastitis in mice via the NFkappaB signalling pathway. Mol Med Rep 19, 2279-2286
28 Weisskopf L, Schulz S and Garbeva P (2021) Microbial volatile organic compounds in intra-kingdom and inter-kingdom interactions. Nat Rev Microbiol 19, 391-404   DOI