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http://dx.doi.org/10.5483/BMBRep.2022.55.6.044

Inhibition of ClC-5 suppresses proliferation and induces apoptosis in cholangiocarcinoma cells through the Wnt/β-catenin signaling pathway  

Shi, Zhe (Department of General Surgery, Affiliated Hospital of Hebei Engineering University)
Zhou, Liyuan (Department of Gynaecology, Affiliated Hospital of Hebei Engineering University)
Zhou, Yan (Department of Nursing, Medical College, Hebei University of Engineering)
Jia, Xiaoyan (Department of General Surgery, Affiliated Hospital of Hebei Engineering University)
Yu, Xiangjun (Department of General Surgery, Affiliated Hospital of Hebei Engineering University)
An, Xiaohong (Department of Hospital Infection-Control, Jize County People's Hospital)
Han, Yanzhen (Department of General Surgery, Affiliated Hospital of Hebei Engineering University)
Publication Information
BMB Reports / v.55, no.6, 2022 , pp. 299-304 More about this Journal
Abstract
Chloride channel-5 (ClC-5), an important branch of the ClC family, is involved in the regulation of the proliferation and cell-fate of a variety of cells, including tumor cells. However, its function in cholangiocarcinoma (CCA) cells remains enigmatic. Here, we discovered that ClC-5 was up-regulated in CCA tissues and CCA cell lines, while ClC-5 silencing inhibited CCA cell proliferation and induced apoptosis. Further mechanism studies revealed that ClC-5 inhibition could inhibit Wnt/β-catenin signaling activity and further activate the mitochondria apoptotic pathway in CCA cells. Furthermore, rescuing Wnt/β-catenin signaling activation eliminated the anti-tumor function of ClC-5 knockdown. Together, our research findings illustrated that ClC-5 inhibition plays an anti-tumor role in CCA cells via inhibiting the activity of the Wnt/β-catenin pathway, which in turn activates the mitochondrial apoptotic pathway.
Keywords
Apoptosis; Chloride channel-5; Cholangiocarcinoma; Proliferation; Wnt/${\beta}$-catenin signaling pathway;
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