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http://dx.doi.org/10.5483/BMBRep.2021.54.8.022

EBP1 regulates Suv39H1 stability via the ubiquitin-proteasome system in neural development  

Kim, Byeong-Seong (Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine)
Ko, Hyo Rim (Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine)
Hwang, Inwoo (Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine)
Cho, Sung-Woo (Department of Biochemistry and Molecular Biology, University of Ulsan, College of Medicine)
Ahn, Jee-Yin (Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine)
Publication Information
BMB Reports / v.54, no.8, 2021 , pp. 413-418 More about this Journal
Abstract
ErbB3-binding protein 1 (EBP1) is a multifunctional protein associated with neural development. Loss of Ebp1 leads to upregulation of the gene silencing unit suppressor of variegation 3-9 homolog 1 (Suv39H1)/DNA (cytosine 5)-methyltransferase (DNMT1). EBP1 directly binds to the promoter region of DNMT1, repressing DNA methylation, and hence, promoting neural development. In the current study, we showed that EBP1 suppresses histone methyltransferase activity of Suv39H1 by promoting ubiquitin-proteasome system (UPS)-dependent degradation of Suv39H1. In addition, we showed that EBP1 directly interacts with Suv39H1, and this interaction is required for recruiting the E3 ligase MDM2 for Suv39H1 degradation. Thus, our findings suggest that EBP1 regulates UPS-dependent degradation of Suv39H1 to govern proper heterochromatin assembly during neural development.
Keywords
ErbB3-binding protein 1; MDM2; Suppressor of variegation 3-9 homolog 1; Ubiquitin-proteasome system;
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