[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.5483/BMBRep.2020.53.8.024

Antibody-secreting macrophages generated using CpG-free plasmid eliminate tumor cells through antibody-dependent cellular phagocytosis

Cha, Eun Bi (Environmental Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Shin, Keun Koo (Environmental Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Seo, Jinho (Environmental Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Oh, Doo-Byoung (Environmental Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))

Publication Information

BMB Reports / v.53, no.8, 2020 , pp. 442-447 More about this Journal

Abstract

The non-viral delivery of genes into macrophages, known as hard-to-transfect cells, is a challenge. In this study, the microporation of a CpG-free and small plasmid (pCGfd-GFP) showed high transfection efficiency, sustainable transgene expression, and good cell viability in the transfections of Raw 264.7 and primary bone marrow-derived macrophages. The non-viral method using the pCGfd vector encoding anti-EGFR single-chain Fv fused with Fc (scFv-Fc) generated the macrophages secreting anti-EGFR scFv-Fc. These macrophages effectively phagocytized tumor cells expressing EGFR through the antibody-dependent mechanism, as was proved by experiments using EGFR-knockout tumor cells. Finally, peri-tumoral injections of anti-EGFR scFv-Fc-secreting macrophages were shown to inhibit tumor growth in the xenograft mouse model.

Keywords

Antibody-dependent cellular phagocytosis; Antibody-secreting macrophage; CpG-free plasmid; Macrophages; Non-viral gene delivery;

Citations & Related Records

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