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http://dx.doi.org/10.5483/BMBRep.2020.53.5.219

MiR-449a attenuates autophagy of T-cell lymphoma cells by downregulating ATG4B expression  

Zhang, Nan (Department of Hematology, The General Hospital of Western Theater Command)
Qiu, Ling (Department of Hematology, The General Hospital of Western Theater Command)
Li, Tao (Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Army Medical University)
Wang, Xiao (Department of Hematology, The General Hospital of Western Theater Command)
Deng, Rui (Department of Hematology, The General Hospital of Western Theater Command)
Yi, Hai (Department of Hematology, The General Hospital of Western Theater Command)
Su, Yi (Department of Hematology, The General Hospital of Western Theater Command)
Fan, Fang-yi (Department of Hematology, The General Hospital of Western Theater Command)
Publication Information
BMB Reports / v.53, no.5, 2020 , pp. 254-259 More about this Journal
Abstract
Increasing evidence suggests the role of miR-449a in the regulation of tumorigenesis and autophagy. Autophagy plays an important role in the malignancy of T-cell lymphoma. However, it is still unknown whether miR-449a is associated with autophagy to regulate the malignancy of T-cell lymp homa. In this study, we for the first time demonstrated that miR-449a enhanced apoptosis of T-cell lymphoma cells by decreasing the degree of autophagy. Further, miR-449a downregulated autophagy-associated 4B (ATG4B) expression, which subsequently reduced the autophagy of T-cell lymphoma cells. Mechanistically, miR-449a decreased ATG4B protein level by binding to its mRNA 3'UTR, thus reducing the mRNA stability. In addition, studies with nude mice showed that miR-449a significantly inhibited lymphoma characteristics in vivo. In conclusion, our results demonstrated that the "miR-449a/ATG4B/autophagy" pathway played a vital role in the malignancy of T-cell lymphoma, suggesting a novel therapeutic target.
Keywords
ATG4B; Autophagy; MiR-449a; Post-transcriptional regulation; T-cell lymphoma;
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