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http://dx.doi.org/10.5483/BMBRep.2020.53.2.153

Functional analysis of RNA motifs essential for BC200 RNA-mediated translational regulation  

Jang, Seonghui (Department of Chemistry, KAIST)
Shin, Heegwon (Department of Chemistry, KAIST)
Lee, Younghoon (Department of Chemistry, KAIST)
Publication Information
BMB Reports / v.53, no.2, 2020 , pp. 94-99 More about this Journal
Abstract
Brain cytoplasmic 200 RNA (BC200 RNA) is proposed to act as a local translational modulator by inhibiting translation after being targeted to neuronal dendrites. However, the mechanism by which BC200 RNA inhibits translation is not fully understood. Although a detailed functional analysis of RNA motifs is essential for understanding the BC200 RNA-mediated translation-inhibition mechanism, there is little relevant research on the subject. Here, we performed a systematic domain-dissection analysis of BC200 RNA to identify functional RNA motifs responsible for its translational-inhibition activity. Various RNA variants were assayed for their ability to inhibit translation of luciferase mRNA in vitro. We found that the 111-200-nucleotide region consisting of part of the Alu domain as well as the A/C-rich domain (consisting of both the A-rich and C-rich domains) is most effective for translation inhibition. Surprisingly, we also found that individual A-rich, A/C-rich, and Alu domains can enhance translation but at different levels for each domain, and that these enhancing effects manifest as cap-dependent translation.
Keywords
A/C-rich domain; Alu domain; BC200 RNA; RNA motifs; Translational regulation;
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Times Cited By KSCI : 2  (Citation Analysis)
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