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http://dx.doi.org/10.5483/BMBRep.2019.52.6.166

CCAAT/enhancer-binding protein beta (C/EBPβ) is an important mediator of 1,25 dihydroxyvitamin D3 (1,25D3)-induced receptor activator of nuclear factor kappa-B ligand (RANKL) expression in osteoblasts  

Jo, Sungsin (Hanyang University Hospital for Rheumatic Diseases)
Lee, Yun Young (Department of Biomedical Sciences, Graduate School of Biomedical Science and Engineering, Hanyang University)
Han, Jinil (Hanyang University Hospital for Rheumatic Diseases)
Lee, Young Lim (Hanyang University Hospital for Rheumatic Diseases)
Yoon, Subin (Hanyang University Hospital for Rheumatic Diseases)
Lee, Jaehyun (Hanyang University Hospital for Rheumatic Diseases)
Oh, Younseo (Hanyang University Hospital for Rheumatic Diseases)
Han, Joong-Soo (Biomedical Research Institute and Department of Biochemistry and Molecular Biology, College of Medicine, Hanyang University)
Sung, Il-Hoon (Department of Orthopedic Surgery, Hanyang University Hospital)
Park, Ye-Soo (Department of Orthopedic Surgery, Hanyang University Hospital)
Kim, Tae-Hwan (Hanyang University Hospital for Rheumatic Diseases)
Publication Information
BMB Reports / v.52, no.6, 2019 , pp. 391-396 More about this Journal
Abstract
Receptor activator of nuclear factor kappa B ligand (RANKL) expression in osteoblasts is regulated by 1,25-dihydroxyvitamin D3 (1,25D3). CCAAT/enhancer-binding protein beta ($C/EBP{\beta}$) has been proposed to function as a transcription factor and upregulate RANKL expression, but it is still uncertain how $C/EBP{\beta}$ is involved in 1,25D3-induced RANKL expression of osteoblasts. 1,25D3 stimulation increased the expression of RANKL and $C/EBP{\beta}$ genes in osteoblasts and enhanced phosphorylation and stability of these proteins. Moreover, induction of RANKL expression by 1,25D3 in osteoblasts was downregulated upon knockdown of $C/EBP{\beta}$. In contrast, $C/EBP{\beta}$ overexpression directly upregulated RANKL promoter activity and exhibited a synergistic effect on 1,25D3-induced RANKL expression. In particular, 1,25D3 treatment of osteoblasts increased $C/EBP{\beta}$ protein binding to the RANKL promoter. In conclusion, $C/EBP{\beta}$ is required for induction of RANKL by 1,25D3.
Keywords
1,25-dihydroxyvitamin D3 (1,25D3); $C/EBP{\beta}$; Osteoblasts; RANKL;
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